CD63 Antibody (YA5577)
(Synonyms: Lysosomal associated membrane protein 3 antibody; CD 63 antibody; CD63 antibody; CD63 antigen; melanoma 1 antigen; antibody; CD63 antigen antibody; CD63 antigen melanoma 1 antigen antibody; CD63 molecule antibody; CD63_HUMAN antibody; gp55 antibody; Granulophysin antibody; LAMP 3 antibody; LAMP-3 antibody; LAMP3 antibody; LIMP antibody; Lysosomal-associated membrane protein 3 antibody; Lysosome associated membrane glycoprotein 3 antibody; Lysosome-associated membrane glycoprotein 3 antibody; Mast cell antigen AD1 antibody; ME491 antibody; Melanoma 1 antigen antibody; Melanoma associated antigen ME491 antibody; Melanoma associated antigen MLA1 antibody; Melanoma-associated antigen ME491 antibody; MGC72893 antibody; MLA 1 antibody; MLA1 antibody; NGA antibody; Ocular melanoma associated antigen antibody; Ocular melanoma-associated antigen antibody; OMA81H antibody; PTLGP40 antibody; Tetraspanin 30 antibody; Tetraspanin-30 antibody; Tspan 30 antibody; Tspan-30 antibody; TSPAN30 antibody; )
Based on 1 Customer Validation
CD63 Antibody (YA5577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:250 | 1:1000 | 1-2μg/sample | 1:200 |
Product Details
CD63 Antibody (YA5577) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD63.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 30-65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Recombinant protein within Human CD63 aa 103-203.
Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD63 is a CD63, as a cell surface receptor for TIMP1, participates in the activation of the cell signaling cascade. CD63 participates in the activation of ITGB1 and integrin signals, thereby activating AKT, FAK/PTK2 and MAP kinases. CD63 promotes cell survival, actin cytoskeleton remodeling, cell adhesion, spreading and migration by activating AKT and FAK/PTK2. CD63 participates in the VEGFA signaling pathway by regulating the endocytosis of KDR/VEGFR2. CD63 participates in intracellular vesicle transport and is essential for the normal transport of the PMEL luminal domain, which is crucial for the development and maturation of melanocytes. CD63 participates in the adhesion of leukocytes to endothelial cells by regulating the transport of SELP. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but does not play a role in degranulation in response to other stimuli[1].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Endosome, multivesicular body; Melanosome; Secreted, extracellular exosome; Cell surface
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Expression
Tissue_specificity:Detected in platelets (protein level) . Dysplastic nevi, primary melanoma in the radial growth phase, hematopoietic cells, tissue macrophages. -
Isoforms & Post-Translational Modification
CD63 has 3 isomers: P08962-1: 25637 Da (predicted); P08962-2: 23430 Da (predicted); P08962-3: 17271 Da (predicted).
Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) -
Subunit
Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1 (PubMed:16917503, PubMed:24635319). Interacts with CD9. Identified in a complex with CD9 and ITGB3 (PubMed:19640571). Interacts with PMEL (PubMed:21962903). Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes (PubMed:23632027). Interacts with SYT7 (By similarity)
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SwissProt ID
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Synonyms
Lysosomal associated membrane protein 3 antibody; CD 63 antibody; CD63 antibody; CD63 antigen; melanoma 1 antigen; antibody; CD63 antigen antibody; CD63 antigen melanoma 1 antigen antibody; CD63 molecule antibody; CD63_HUMAN antibody; gp55 antibody; Granulophysin antibody; LAMP 3 antibody; LAMP-3 antibody; LAMP3 antibody; LIMP antibody; Lysosomal-associated membrane protein 3 antibody; Lysosome associated membrane glycoprotein 3 antibody; Lysosome-associated membrane glycoprotein 3 antibody; Mast cell antigen AD1 antibody; ME491 antibody; Melanoma 1 antigen antibody; Melanoma associated antigen ME491 antibody; Melanoma associated antigen MLA1 antibody; Melanoma-associated antigen ME491 antibody; MGC72893 antibody; MLA 1 antibody; MLA1 antibody; NGA antibody; Ocular melanoma associated antigen antibody; Ocular melanoma-associated antigen antibody; OMA81H antibody; PTLGP40 antibody; Tetraspanin 30 antibody; Tetraspanin-30 antibody; Tspan 30 antibody; Tspan-30 antibody; TSPAN30 antibody;
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