Histone H4 (Tri Methyl Lys20) Antibody (YA7527)

Cat. No.: HY-P87842: Fiche technique COA
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Histone H4 (Tri Methyl Lys20) Antibody (YA7527) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H4 (Tri Methyl Lys20).

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

Histone H4 (Tri Methyl Lys20) Antibody (YA7527) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H4 (Tri Methyl Lys20).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Masse moléculaire

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P62805

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200
CHIP	1:50-1:100
Cut&Tag 1	50-1:100

Sensitivity	Endogenous	Pureté	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Methylated
Isotype	IgG,Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Subcellular Localization：Nucleus,Chromosome

Isoforms & Post-Translational Modification：P62805: 103 amino acids, molecular weight 11367 Da.

Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers

Documentation

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Fiche technique (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)