ATG12 Antibody (YA6468)
(Synonyms: Apg12 antibody; APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; Apg12 antibody; APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin-like protein ATG12 antibody; )ATG12 Antibody (YA6468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG12.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:250 | 1:500-1:1000 |
Product Details
ATG12 Antibody (YA6468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG12.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 55/20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 15 kDa
Synthetic peptide within Human ATG12 aa 1-50 / 140.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ATG12 is an Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress|(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established[1][2].
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Subcellular Localization
Cytoplasm,Preautophagosomal structure membrane
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Expression
Tissue_Specificity: Ubiquitous
Induction: Expression is induced by mitochondrial DNA deletions, chloramphenicol and nicotinamide -
Isoforms & Post-Translational Modification
O94817 has two isomers: O94817-1: 15113 Da (predicted); O94817-4: 7746 Da (predicted).
Acetylated by EP300 -
Subunit
Forms a conjugate with ATG5 (PubMed:11096062, PubMed:11825910, PubMed:12207896, PubMed:17709747, PubMed:23202584, PubMed:24191030, PubMed:26812546)
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SwissProt ID
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Synonyms
Apg12 antibody; APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; Apg12 antibody; APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin-like protein ATG12 antibody;
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