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FRA1 Antibody (YA2027)

(Synonyms: FOSL1; FRA1; Fos-related antigen 1; FRA-1)
Customer Review

Based on 1 Customer Validation

FRA1 Antibody (YA2027) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FRA1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • FRA1 Antibody (YA2027) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

FRA1 Antibody (YA2027) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FRA1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database

Entrez Gene: 8061 Human

SwissProt: P15407 Human

Immunogen

A synthesized peptide derived from human FRA1 aa130-210.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104394

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FRA1 Antibody (YA2027)
    Western blot analysis of extracts from HL-60(lane 2(20ug) ,A431(lane 3(20ug)and U-87MG(lane 4(20ug) using FRA1 Antibody (HY-P82282) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FRA1 Antibody (YA2027)
    Immunocytochemistry analysis of Hela cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FRA1 Antibody (YA2027)
    Immunocytochemistry analysis of U87 cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    FRA1, a member of the FOS family, as a critical transcription factor during acinar de-differentiation to ADM. FRA1 depletion leads to resistance to ADM and PanIN formation after induction of acute pancreatitis. Mechanistically, FRA1 serves a protective role in acinar cells by preventing cell death and aiding in the progression of the ADM-PanIN-carcinoma continuum[1].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    FRA1 has 2 isomers: P15407-1: 29413 Da (predicted); P15407-2: 17560 Da (predicted).

  • Subunit

    Heterodimer (By similarity).

  • SwissProt ID

    P15407

  • Gene ID
    8061 [NCBI]
  • Synonyms

    FOSL1; FRA1; Fos-related antigen 1; FRA-1

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Li AL, et al. FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia. Dev Cell. 2024 Nov 18;59(22):3025-3042.e7. [Content Brief]

FRA1 Antibody (YA2027) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) ,A431(lane 3(20ug)and U-87MG(lane 4(20ug) using FRA1 Antibody (HY-P82282) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FRA1 Antibody (YA2027) Product Image
    1/16
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • FRA1 Antibody (YA2027) Product Image
  • Experimental Validation Results for FRA1 Antibody (YA2027)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) ,A431(lane 3(20ug)and U-87MG(lane 4(20ug) using FRA1 Antibody (HY-P82282) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FRA1 Antibody (YA2027)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FRA1 Antibody (YA2027)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U87 cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • Experimental Validation Results for FRA1 Antibody (YA2027)
  • FRA1 Antibody (YA2027) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL-60(lane 2(20ug) ,A431(lane 3(20ug)and U-87MG(lane 4(20ug) using FRA1 Antibody (HY-P82282) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FRA1 Antibody (YA2027) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FRA1 Antibody (YA2027) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U87 cells labeling FRA1 with FRA1 Antibody (HY-P82282) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FRA1 Antibody (HY-P82282) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FRA1 Antibody (YA2027) Product Image WB
  • FRA1 Antibody (YA2027) Product Image ICC
  • FRA1 Antibody (YA2027) Product Image ICC