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  2. Primary Antibodies
  3. Phospho-CDC37 (Ser13) Antibody (YA219)

Phospho-CDC37 (Ser13) Antibody (YA219)

Cat. No.: HY-P80962
COA User Guide for Antibodies

Phospho-CDC37 (Ser13) Antibody (YA219) is a non-conjugated and Rabbit origined monoclonal antibody about 44 kDa, targeting to Hsp90 co-chaperone Cdc37 (CDC37). It can be used for WB,IP assays in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $90 In-stock
50 μL $230 In-stock
100 μL $370 In-stock
250 μL   Get quote  

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  • Biological Activity

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Description

Phospho-CDC37 (Ser13) Antibody (YA219) is a non-conjugated and Rabbit origined monoclonal antibody about 44 kDa, targeting to Hsp90 co-chaperone Cdc37 (CDC37). It can be used for WB,IP assays in the background of Human, Mouse, Rat.

Background

Hsp90 co-chaperone Cdc37 (CDC37): The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]

Gene ID

11140

Molecular Weight

Predicted band size: 44 kDa

Conjugation

Non-conjugated

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

SwissProt ID

Q16543

Dilution Ratio

WB: 1:500-1:1000 ;IP: 1:20

  • Western blot analysis of extracts from Hela(lane 2(20μg) ) and Hela(lane 3(20μg), Calyculin A treated (HY-18983, 50nm, 3hrs) as indicated, using Phospho-CDC37 (Ser13) (HY-P80962) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80962, 1/1000) and Loading control antibody (CDC37, HY-P80960, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IP

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser13 of Human Cdc37.The exact sequence is proprietary to MCE.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phospho-CDC37 (Ser13) Antibody (YA219)
Cat. No.:
HY-P80962
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