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CYFIP1 Antibody (YA2605)

(Synonyms: SHYC; SRA1; SRA-1; P140SRA-1)
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Based on 1 Customer Validation

CYFIP1 Antibody (YA2605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CYFIP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CYFIP1 Antibody (YA2605)
  • CYFIP1 Antibody (YA2605) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

CYFIP1 Antibody (YA2605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CYFIP1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 145 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 145 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human CYFIP1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104953

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CYFIP1 Antibody (YA2605)
    Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using CYFIP1 Antibody (HY-P82860) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    CYFIP1 component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA. Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. May act as an invasion suppressor in cancers

  • Subcellular Localization

    Cytoplasm; Cytoplasm, perinuclear region; Cell projection, lamellipodium; Cell projection, ruffle; Synapse, synaptosome

  • Isoforms & Post-Translational Modification

    Q7L576 has 3 isomers: Q7L576-1: 145182 Da (predicted); Q7L576-2: 94467 Da (predicted); Q7L576-3: 51534 Da (predicted).

  • Subunit

    Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with FMR1 but does not bind to related proteins FXR1 or FXR2. Interaction with EIF4E stimulates FMR1 binding. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2 (PubMed:16417406). Interacts with the active GTP-bound form of RAC1. Interacts through its C-terminus with the C-terminus of DPYSL2/CRMP2 which is necessary for DPYSL2-induced axon outgrowth. Interacts with NYAP1, NYAP2 and MYO16. Interacts with TMEM108 (via N-terminus); the interaction associates TMEM108 with the WAVE1 complex (By similarity)

  • SwissProt ID

    Q7L576

  • Gene ID
    23191 [NCBI]
  • Synonyms

    SHYC; SRA1; SRA-1; P140SRA-1

  • Research Field

    -

CYFIP1 Antibody (YA2605) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using CYFIP1 Antibody (HY-P82860) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CYFIP1 Antibody (YA2605) Product Image
    1/16
  • Experimental Validation Results for CYFIP1 Antibody (YA2605)
  • CYFIP1 Antibody (YA2605) Product Image
  • Experimental Validation Results for CYFIP1 Antibody (YA2605)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using CYFIP1 Antibody (HY-P82860) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CYFIP1 Antibody (YA2605)
  • CYFIP1 Antibody (YA2605) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using CYFIP1 Antibody (HY-P82860) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CYFIP1 Antibody (YA2605) Product Image WB