EGF Antibody (YA469)

Cat. No.: HY-P80115: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

EGF Antibody (YA469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGF.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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EGF Antibody (YA469) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EGF Antibody (YA469) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGF.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 134 kDa;

Observed band size: 160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P01133

Gene ID

1950 [NCBI]

Immunogen

Synthetic peptide corresponding to Human EGF.AA range:201-350.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:10-1:50

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941)

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is expressed in the kidneys, salivary glands, brain, and prostate.

Isoforms & Post-Translational Modification：P01133 has 3 isomers: P01133-1: 133994 Da (predicted); P01133-2: 129256 Da (predicted); P01133-3: 129746 Da (predicted).
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor)

Subunit：Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2 (By similarity).

RRID

AB_3102827

Database

Entrez Gene: 1950 Human

SwissProt: P01133 Human

OMIM: 611718 Human

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

EGF Antibody (YA469) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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EGF Antibody (YA469)

EGF Antibody (YA469) Featured Recommendations:

Product Detail

Background

Documentation

EGF Antibody (YA469) Related Classifications

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