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Osteoprotegerin Antibody (YA3535)

(Synonyms: MGC29565 antibody; OCIF antibody; OPG antibody; Osteoclastogenesis inhibitory factor antibody; Osteoprotegerin antibody; PDB5 antibody; TNF receptor superfamily member 11b antibody; TNFRSF 11B antibody; TNFRSF11B antibody; TR 1 antibody; TR1 antibody; TR11B_HUMAN antibody; Tumor necrosis factor receptor superfamily member 11B antibody; )
Customer Review

Based on 1 Customer Validation

Osteoprotegerin Antibody (YA3535) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Osteoprotegerin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Osteoprotegerin Antibody (YA3535) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:200

Product Details

Description

Osteoprotegerin Antibody (YA3535) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Osteoprotegerin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

Recombinant protein within Human Osteoprotegerin aa 1-401 / 401.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103224

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg) using Osteoprotegerin (HY-P81123) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Osteoprotegerin (OPG) belongs to TNF receptor superfamily. OPG is expressed in many tissues besides osteoblasts, including heart, kidney, liver, spleen, and bone marrow. Osteoprotegerin plays a critical role in bone remodeling, and has osteoprotective effect. Osteoprotegerin also protects large blood vessels from medial calcification. Osteoprotegerin can bind to RANKL and inhibit the binding between TNFSF11 and RANKL, thereby neutralizing the RANKL function in osteoclastogenesis[1]. OPG is also involved in multiple processes of cancers, such as tumor survival, epithelial to mesenchymal transition (EMT), neo-angiogenesis, invasion, and metastasis. Human osteoprotegerin shares <85% aa sequence identity with mouse and rat. Mouse OX40 shares 94.5% aa sequence identity with rat.

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is highly expressed in human lungs, heart, kidneys, liver, spleen, thymus, prostate, ovaries, small intestine, thyroid gland, lymph nodes, trachea, adrenal glands, testes, and bone marrow. Its expression level is extremely low in the brain, placenta, and skeletal muscle. It is highly expressed in fetal kidneys, liver, and lungs.

    Induction:Up-regulated by increasing calcium-concentration in the medium and estrogens. Down-regulated by glucocorticoids

  • Subunit

    Homodimer. Interacts with TNFSF10 and TNFSF11

  • SwissProt ID

    O00300

  • Gene ID
    4982 [NCBI]
  • Synonyms

    MGC29565 antibody; OCIF antibody; OPG antibody; Osteoclastogenesis inhibitory factor antibody; Osteoprotegerin antibody; PDB5 antibody; TNF receptor superfamily member 11b antibody; TNFRSF 11B antibody; TNFRSF11B antibody; TR 1 antibody; TR1 antibody; TR11B_HUMAN antibody; Tumor necrosis factor receptor superfamily member 11B antibody;

References

[1]. Boyce BF, et al. Biology of RANK, RANKL, and osteoprotegerin. Arthritis Res Ther. 2007;9 Suppl 1(Suppl 1):S1. [Content Brief]

[2]. Wang Y, et al. The roles of osteoprotegerin in cancer, far beyond a bone player. Cell Death Discov. 2022 May 6;8(1):252. [Content Brief]

Osteoprotegerin Antibody (YA3535) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg) using Osteoprotegerin (HY-P81123) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Osteoprotegerin Antibody (YA3535) Product Image
    1/16
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Osteoprotegerin Antibody (YA3535) Product Image
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg) using Osteoprotegerin (HY-P81123) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Experimental Validation Results for Osteoprotegerin Antibody (YA3535)
  • Osteoprotegerin Antibody (YA3535) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg) using Osteoprotegerin (HY-P81123) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Osteoprotegerin Antibody (YA3535) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Osteoprotegerin Antibody (YA3535) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteoprotegerin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Osteoprotegerin Antibody (YA3535) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Osteoprotegerin Antibody (YA3535) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HEK-293 cells labeling Osteoprotegerin with Osteoprotegerin Antibody (YA3535) (HY-P81123) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Osteoprotegerin Antibody (YA3535) ((HY-P81123) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Osteoprotegerin Antibody (YA3535) Product Image WB
  • Osteoprotegerin Antibody (YA3535) Product Image IHC-P
  • Osteoprotegerin Antibody (YA3535) Product Image IHC-P
  • Osteoprotegerin Antibody (YA3535) Product Image ICC
  • Osteoprotegerin Antibody (YA3535) Product Image ICC