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PSMA Antibody (YA1764)(PBS only)

(Synonyms: FGCP; FOLH 1; GCP 2; GCPII; mGCP; NAALADase I; PSM; PSMA)

PSMA Antibody (YA1764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSMA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PSMA Antibody (YA1764)(PBS only)
  • PSMA Antibody (YA1764)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PSMA Antibody (YA1764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSMA.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Immunogen

A synthetic peptide of human PSMA aa361-374.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PSMA Antibody (YA1764)(PBS only)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using PSMA Antibody (HY-P82019) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PSMA is a transmembrane protein that anchores to the cell membrane through its transmembrane region and is mainly expressed in prostate epithelial cells but also exists in other tissues. PSMA exhibits glutamate-preferring carboxypeptidase activity that hydrolyzes polyglutamylated folic acid to release folic acid monomers. PSMA mediates the endocytosis on the cell surface. PSMA acts as an angiogenesis marker in cancer cells. PSMA can be used as a diagnostic marker for prostate cancer, and for detection of invasiveness and metastatic ability of the tumor[1].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:It is highly expressed in prostate epithelial cells. It can be detected in the bladder, kidneys, testes, ovaries, fallopian tubes, breasts, adrenal glands, liver, esophagus, stomach, small intestine, colon, and brain (protein level) . It can also be detected in the capillary endothelial cells of the small intestine, brain, kidneys, liver, spleen, colon, trachea, spinal cord, and various tumors. It is specifically expressed in the brush border membrane of the jejunum. In the brain, it is highly expressed in the ventral striatum and brainstem. It is also expressed in fetal liver and kidneys. The PSMA' subtype is the most abundant subtype in normal prostate. The PSMA-1 subtype is the most abundant subtype in primary prostate tumors. The PSMA-9 subtype is specifically expressed in prostate cancer.

    Induction:In the prostate, up-regulated in response to androgen deprivation

  • Isoforms & Post-Translational Modification

    Q04609 has 8 isomers: Q04609-1: 84331 Da (predicted); Q04609-3: 78065 Da (predicted); Q04609-4: 27811 Da (predicted); Q04609-6: 78000 Da (predicted); Q04609-7: 82519 Da (predicted); Q04609-8: 80597 Da (predicted); Q04609-9: 78785 Da (predicted); Q04609-10: 50090 Da (predicted).
    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis;The N-terminus is blocked

  • Subunit

    Homodimer

  • SwissProt ID

    Q04609

  • Gene ID
    2346 [NCBI]
  • Synonyms

    FGCP; FOLH 1; GCP 2; GCPII; mGCP; NAALADase I; PSM; PSMA

  • Research Field

    Signal Transduction

References

[1]. Chang SS. Overview of prostate-specific membrane antigen. Rev Urol. 2004;6 Suppl 10(Suppl 10):S13-8. [Content Brief]

PSMA Antibody (YA1764)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using PSMA Antibody (HY-P82019) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PSMA Antibody (YA1764)(PBS only) Product Image
    1/16
  • Experimental Validation Results for PSMA Antibody (YA1764)(PBS only)
  • PSMA Antibody (YA1764)(PBS only) Product Image
  • Experimental Validation Results for PSMA Antibody (YA1764)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using PSMA Antibody (HY-P82019) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PSMA Antibody (YA1764)(PBS only)
  • PSMA Antibody (YA1764)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) and NIH/3T3(lane 3(40ug) using PSMA Antibody (HY-P82019) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PSMA Antibody (YA1764)(PBS only) Product Image WB