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IGF2BP2 Antibody (YA2033)

(Synonyms: IGF2BP2; IMP2; VICKZ2; Insulin-like growth factor 2 mRNA-binding protein 2; IGF2 mRNA-binding protein 2; IMP-2; Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2)
Customer Review

Based on 1 Customer Validation

IGF2BP2 Antibody (YA2033) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGF2BP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • IGF2BP2 Antibody (YA2033) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

IGF2BP2 Antibody (YA2033) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGF2BP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 66,62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human IMP2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104400

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using IGF2BP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Western blot analysis of extracts from SY-SY5Y(lane 2(20ug) ,HEK293(lane 3(20ug) and Jurkat(lane 4(20ug) using IGF2BP2 Antibody (HY-P82288) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Stromal tumor‌ tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Thyroid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunohistochemical analysis of paraffin-embedded human Squamous Cell Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    IGF2BP2 is a RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD[1][2].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, P-body; Cytoplasm, Stress granule

  • Expression


    Tissue_specificity:Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level) . Expressed in testicular cancer (at protein level) . Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis

  • Isoforms & Post-Translational Modification

    Q9Y6M1 has 6 isomers: Q9Y6M1-2: 66121 Da (predicted); Q9Y6M1-1: 61842 Da (predicted); Q9Y6M1-3: 59001 Da (predicted); Q9Y6M1-4: 59666 Da (predicted); Q9Y6M1-5: 54722 Da (predicted); Q9Y6M1-6: 58578 Da (predicted).

  • Subunit

    Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner (PubMed:23640942). Interacts with HNRPD (PubMed:12674497). Interacts with IGF2BP1 (PubMed:17289661). Interacts with ELAVL1, DHX9, HNRNPU, MATR3 and PABPC1 (PubMed:23640942, PubMed:29476152). Interacts with the HOXB-AS3 peptide; the interaction increases MYC stability (PubMed:34457052)

  • SwissProt ID

    Q9Y6M1

  • Gene ID
    10644 [NCBI]
  • Synonyms

    IGF2BP2; IMP2; VICKZ2; Insulin-like growth factor 2 mRNA-binding protein 2; IGF2 mRNA-binding protein 2; IMP-2; Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2

  • Research Field

    Epigenetics and Nuclear Signaling

IGF2BP2 Antibody (YA2033) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using IGF2BP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SY-SY5Y(lane 2(20ug) ,HEK293(lane 3(20ug) and Jurkat(lane 4(20ug) using IGF2BP2 Antibody (HY-P82288) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Stromal tumor‌ tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Squamous Cell Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF2BP2 Antibody (YA2033) Product Image
    1/16
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • IGF2BP2 Antibody (YA2033) Product Image
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using IGF2BP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SY-SY5Y(lane 2(20ug) ,HEK293(lane 3(20ug) and Jurkat(lane 4(20ug) using IGF2BP2 Antibody (HY-P82288) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Stromal tumor‌ tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Squamous Cell Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • Experimental Validation Results for IGF2BP2 Antibody (YA2033)
  • IGF2BP2 Antibody (YA2033) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), and Rat brain (lane 2, 15 μg) using IGF2BP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SY-SY5Y(lane 2(20ug) ,HEK293(lane 3(20ug) and Jurkat(lane 4(20ug) using IGF2BP2 Antibody (HY-P82288) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Stromal tumor‌ tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human serous ovarian cancer tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Uterine leiomyoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Squamous Cell Carcinoma tissue using IGF2BP2 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82288, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF2BP2 Antibody (YA2033) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling IGF2BP2 Antibody(HY-P82288) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with IGF2BP2 Antibody(HY-P82288) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF2BP2 Antibody (YA2033) Product Image WB
  • IGF2BP2 Antibody (YA2033) Product Image WB
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image IHC-P
  • IGF2BP2 Antibody (YA2033) Product Image ICC
  • IGF2BP2 Antibody (YA2033) Product Image ICC