Nectin-4 Antibody (YA6688)

Cat. No.: HY-P86995: Data Sheet COA
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Nectin-4 Antibody (YA6688) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nectin-4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Nectin-4 Antibody (YA6688) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nectin-4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 45-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96NY8

Gene ID

81607 [NCBI]

Immunogen

Recombinant protein within human Nectin-4 aa 1-400.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Raji (lane 3(20μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Human breast cancer (lane 1(40μg)) 、Human liver cancer (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells|(Microbial infection) Acts as a receptor for measles virus

Subcellular Localization：Cell membrane,Cell junction, adherens junction,Secreted

Expression：
Tissue_Specificity: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma

Isoforms & Post-Translational Modification：Q96NY8 has two isomers: Q96NY8-1: 55454 Da (predicted); Q96NY8-2: 24341 Da (predicted).
The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE

Subunit：Self-associates

Synonyms

DKFZp686K05193 antibody; EDSS1 antibody; Ig superfamily receptor LNIR antibody; Nectin 4 antibody; Nectin-4 antibody; poliovirus receptor-related 4 antibody; Processed poliovirus receptor-related protein 4 antibody; PRR4 antibody; pvrl4 antibody; PVRL4_HUMAN antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)