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Nectin-4 Antibody (YA6688)

(Synonyms: DKFZp686K05193 antibody; EDSS1 antibody; Ig superfamily receptor LNIR antibody; Nectin 4 antibody; Nectin-4 antibody; poliovirus receptor-related 4 antibody; Processed poliovirus receptor-related protein 4 antibody; PRR4 antibody; pvrl4 antibody; PVRL4_HUMAN antibody; )
Customer Review

Based on 1 Customer Validation

Nectin-4 Antibody (YA6688) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nectin-4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Nectin-4 Antibody (YA6688) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:200-1:1000

Product Details

Description

Nectin-4 Antibody (YA6688) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nectin-4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45-70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Recombinant protein within human Nectin-4 aa 1-400.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Raji (lane 3(20μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    Western blot analysis of extracts from Human breast cancer (lane 1(40μg)) 、Human liver cancer (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    Nectin-4 Antibody (HY-P86995) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4（HY-P70616U） with an affinity constant of 4.49 nM as determined in a SPR assay.(Biacore 8K).

Background

  • Function

    Nectin-4 is a cell adhesion molecule that plays a crucial role in cell-cell adhesion and communication. Nectin-4 is involved in various physiological processes such as embryonic development and tissue homeostasis. Nectin-4 is overexpressed on the surface of tumor cells, making it an attractive target for cancer therapy. Nectin-4 belongs to the nectin family of proteins, which is characterized by immunoglobulin-like domains and plays important roles in mediating cell-cell interactions through homophilic and heterophilic binding[1][2][3].

  • Subcellular Localization

    Cell membrane,Cell junction, adherens junction,Secreted

  • Expression


    Tissue_Specificity: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma

  • Isoforms & Post-Translational Modification

    Q96NY8 has two isomers: Q96NY8-1: 55454 Da (predicted); Q96NY8-2: 24341 Da (predicted).
    The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE

  • Subunit

    Self-associates

  • SwissProt ID

    Q96NY8

  • Gene ID
    81607 [NCBI]
  • Synonyms

    DKFZp686K05193 antibody; EDSS1 antibody; Ig superfamily receptor LNIR antibody; Nectin 4 antibody; Nectin-4 antibody; poliovirus receptor-related 4 antibody; Processed poliovirus receptor-related protein 4 antibody; PRR4 antibody; pvrl4 antibody; PVRL4_HUMAN antibody;

References

[1]. Liu Y, et al. Role of Nectin?4 protein in cancer (Review). Int J Oncol. 2021 Nov;59(5):93. [Content Brief]

[2]. Heath EI, et al. The biology and rationale of targeting nectin-4 in urothelial carcinoma. Nat Rev Urol. 2021 Feb;18(2):93-103. [Content Brief]

[3]. Hashimoto H, et al. Nectin-4: a Novel Therapeutic Target for Skin Cancers. Curr Treat Options Oncol. 2022 Apr;23(4):578-593. [Content Brief]

Nectin-4 Antibody (YA6688) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Raji (lane 3(20μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Human breast cancer (lane 1(40μg)) 、Human liver cancer (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Nectin-4 Antibody (HY-P86995) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4（HY-P70616U） with an affinity constant of 4.49 nM as determined in a SPR assay.(Biacore 8K).

  • Nectin-4 Antibody (YA6688) Product Image
    1/16
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Nectin-4 Antibody (YA6688) Product Image
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Raji (lane 3(20μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Human breast cancer (lane 1(40μg)) 、Human liver cancer (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
    1/1
    MedChemExpress Validation
     Nectin-4 Antibody (HY-P86995) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4（HY-P70616U） with an affinity constant of 4.49 nM as determined in a SPR assay.(Biacore 8K).
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Experimental Validation Results for Nectin-4 Antibody (YA6688)
  • Nectin-4 Antibody (YA6688) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Raji (lane 3(20μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Nectin-4 Antibody (YA6688) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Human breast cancer (lane 1(40μg)) 、Human liver cancer (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using Nectin-4 Antibody (HY-P86995) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Nectin-4 Antibody (YA6688) Product Image
    MedChemExpress Validation

    Nectin-4 Antibody (HY-P86995) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4（HY-P70616U） with an affinity constant of 4.49 nM as determined in a SPR assay.(Biacore 8K).

  • Nectin-4 Antibody (YA6688) Product Image WB
  • Nectin-4 Antibody (YA6688) Product Image WB
  • Nectin-4 Antibody (YA6688) Product Image