IREB2 Antibody (YA7008)

Cat. No.: HY-P87325: Data Sheet COA
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IREB2 Antibody (YA7008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IREB2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

IREB2 Antibody (YA7008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IREB2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 105 kDa;

Observed band size: 105 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P48200

Immunogen

Recombinant protein within human IREB2 aa 1-963.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from Mouse liver (lane 1), Rat liver (lane 2), Mouse kidney (lane 3), and Mouse lung (lane 4, Negative) using IREB2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA

Subcellular Localization：Cytoplasm

Isoforms & Post-Translational Modification：P48200 has two isomers: P48200-1: 105059 Da (predicted); P48200-2: 37501 Da (predicted).
Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen

Subunit：Interacts with RBCK1 isoform 1 and isoform 2 only in iron-rich conditions (PubMed:12629548)

Synonyms

ACO3 antibody; FLJ23381 antibody; IRE BP2 antibody; IRE-BP 2 antibody; IREB 2 antibody; IREB2 antibody; IREB2_HUMAN antibody; Iron regulatory protein 2 antibody; iron responsive element binding protein 2 antibody; Iron-responsive element-binding protein 2 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)