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Cytokeratin 7 Antibody (YA3718)

(Synonyms: KRT7; cytokeratin 7)
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Based on 1 Customer Validation

Cytokeratin 7 Antibody (YA3718) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytokeratin 7 Antibody (YA3718)
  • Cytokeratin 7 Antibody (YA3718) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Cytokeratin 7 Antibody (YA3718) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Immunogen

Purified recombinant fragment of human CK7 aa 88-222.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in ascitic fluid containing 0.03% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytokeratin 7 Antibody (YA3718)
    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), A431 (lane4, 20μg) and SK-OV-3 (lane5, 20μg) using Cytokeratin 7 Antibody (HY-P84021). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cytokeratin 7 is a Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7)

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:It is expressed in cultured epidermal cells, bronchial cells, and mesothelial cells, but not in the colon, external os of the cervix, or liver. It is also visible in glandular cells at the gastroesophageal junction, but not in the esophagus.

    Induction:Up-regulated by retinoic acid

  • Subunit

    Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10. Interacts with GPER1

  • SwissProt ID

    P08729

  • Gene ID
    3855 [NCBI]
  • Synonyms

    KRT7; cytokeratin 7

Cytokeratin 7 Antibody (YA3718) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), A431 (lane4, 20μg) and SK-OV-3 (lane5, 20μg) using Cytokeratin 7 Antibody (HY-P84021). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Cytokeratin 7 Antibody (YA3718) Product Image
    1/16
  • Experimental Validation Results for Cytokeratin 7 Antibody (YA3718)
  • Cytokeratin 7 Antibody (YA3718) Product Image
  • Experimental Validation Results for Cytokeratin 7 Antibody (YA3718)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), A431 (lane4, 20μg) and SK-OV-3 (lane5, 20μg) using Cytokeratin 7 Antibody (HY-P84021). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytokeratin 7 Antibody (YA3718)
  • Cytokeratin 7 Antibody (YA3718) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg), A431 (lane4, 20μg) and SK-OV-3 (lane5, 20μg) using Cytokeratin 7 Antibody (HY-P84021). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Cytokeratin 7 Antibody (YA3718) Product Image WB