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EPCR Antibody (YA6555)

(Synonyms: Activated protein C receptor antibody; APC receptor antibody; APCR antibody; bA42O4.2 antibody; CCCA antibody; CCD41 antibody; CD201 antibody; CD201 antigen antibody; cell cycle, centrosome-associated protein antibody; Cell cycle, centrosome-associated protein antibody; Activated protein C receptor antibody; APC receptor antibody; APCR antibody; bA42O4.2 antibody; CCCA antibody; CCD41 antibody; CD201 antibody; CD201 antigen antibody; cell cycle, centrosome-associated protein antibody; Cell cycle, centrosome-associated protein antibody; centrocyclin antibody; Endothelial cell protein C receptor antibody; Endothelial protein C receptor antibody; EPCR antibody; EPCR_HUMAN antibody; MGC23024 antibody; PROCR antibody; Protein C receptor, endothelial antibody; )
Customer Review

Based on 1 Customer Validation

EPCR Antibody (YA6555) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EPCR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • EPCR Antibody (YA6555) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:1000

Product Details

Description

EPCR Antibody (YA6555) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EPCR.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

Recombinant protein within human EPCR aa 1-238 / 238.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719224

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EPCR Antibody (YA6555)
    Western blot analysis of extracts from HUVEC (lane2(20μg), Raji (lane3(20μg), HepG2 (lane4(20μg) and Jurkat (lane5(20μg) using EPCR Antibody (HY-P86862). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPCR Antibody (YA6555)
    Immunocytochemistry analysis of Hela cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPCR Antibody (YA6555)
    Immunocytochemistry analysis of PC-3 cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    EPCR binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation

  • Subcellular Localization

    Membrane

  • Expression


    Tissue_Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney

  • Isoforms & Post-Translational Modification

    Q9UNN8: 238 amino acids, molecular weight 26671 Da.
    Autophosphorylated at Ser-1292; autophosphorylation is stimulated by RAB29 (PubMed:28202711, PubMed:28720718, PubMed:29127255, PubMed:29212815, PubMed:30635421, PubMed:38127736)丨Ubiquitinated by TRIM1; undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation

  • SwissProt ID

    Q9UNN8

  • Gene ID
    10544 [NCBI]
  • Synonyms

    Activated protein C receptor antibody; APC receptor antibody; APCR antibody; bA42O4.2 antibody; CCCA antibody; CCD41 antibody; CD201 antibody; CD201 antigen antibody; cell cycle, centrosome-associated protein antibody; Cell cycle, centrosome-associated protein antibody; Activated protein C receptor antibody; APC receptor antibody; APCR antibody; bA42O4.2 antibody; CCCA antibody; CCD41 antibody; CD201 antibody; CD201 antigen antibody; cell cycle, centrosome-associated protein antibody; Cell cycle, centrosome-associated protein antibody; centrocyclin antibody; Endothelial cell protein C receptor antibody; Endothelial protein C receptor antibody; EPCR antibody; EPCR_HUMAN antibody; MGC23024 antibody; PROCR antibody; Protein C receptor, endothelial antibody;

EPCR Antibody (YA6555) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane2(20μg), Raji (lane3(20μg), HepG2 (lane4(20μg) and Jurkat (lane5(20μg) using EPCR Antibody (HY-P86862). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPCR Antibody (YA6555) Product Image
    1/16
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • EPCR Antibody (YA6555) Product Image
  • Experimental Validation Results for EPCR Antibody (YA6555)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane2(20μg), Raji (lane3(20μg), HepG2 (lane4(20μg) and Jurkat (lane5(20μg) using EPCR Antibody (HY-P86862). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPCR Antibody (YA6555)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPCR Antibody (YA6555)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • Experimental Validation Results for EPCR Antibody (YA6555)
  • EPCR Antibody (YA6555) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVEC (lane2(20μg), Raji (lane3(20μg), HepG2 (lane4(20μg) and Jurkat (lane5(20μg) using EPCR Antibody (HY-P86862). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • EPCR Antibody (YA6555) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPCR Antibody (YA6555) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling EPCR with EPCR Antibody (HY-P86862) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPCR Antibody (HY-P86862) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPCR Antibody (YA6555) Product Image WB
  • EPCR Antibody (YA6555) Product Image ICC
  • EPCR Antibody (YA6555) Product Image ICC