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CCR3/CD193 Antibody (YA7736)

(Synonyms: CD193, CMKBR3, CCR3, C-C chemokine receptor type 3, C C CKR3, C-C CKR-3, CC-CKR-3, CCR-3, CKR 3, CKR3, Eosinophil eotaxin receptor)
Customer Review

Based on 1 Customer Validation

CCR3/CD193 Antibody (YA7736) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR3/CD193.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CCR3/CD193 Antibody (YA7736)
  • CCR3/CD193 Antibody (YA7736) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

CCR3/CD193 Antibody (YA7736) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR3/CD193.

  • Host Rabbit
  • Clonality Recombinant
  • Species Reactivity
    Human Predicted Reactivity: Mouse,Rat
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50-60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Immunogen

A synthesized peptide derived from human CCR3: 300-355.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CCR3/CD193 Antibody (YA7736)
    Western blot analysis was performed on protein extracts (40 μg) from HepG2 (lane 2), A549 (lane 3), U87 (lane 4), and Raji (lane 5) using CCR3/CD193 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CCR3/CD193 receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15. Subsequently transduces a signal by increasing the intracellular calcium ions level. In addition acts as a possible functional receptor for NARS1 |(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection[1][2][3][4].

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: In eosinophils as well as trace amounts in neutrophils and monocytes

  • Isoforms & Post-Translational Modification

    P51677 has two isomers: P51677-1: 41044 Da (predicted); P51677-2: 43453 Da (predicted).

  • Subunit

    (Microbial infection) Interacts with HIV-1 protein Tat

  • SwissProt ID

    P51677

  • Synonyms

    CD193, CMKBR3, CCR3, C-C chemokine receptor type 3, C C CKR3, C-C CKR-3, CC-CKR-3, CCR-3, CKR 3, CKR3, Eosinophil eotaxin receptor

CCR3/CD193 Antibody (YA7736) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from HepG2 (lane 2), A549 (lane 3), U87 (lane 4), and Raji (lane 5) using CCR3/CD193 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CCR3/CD193 Antibody (YA7736) Product Image
    1/16
  • Experimental Validation Results for CCR3/CD193 Antibody (YA7736)
  • CCR3/CD193 Antibody (YA7736) Product Image
  • Experimental Validation Results for CCR3/CD193 Antibody (YA7736)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from HepG2 (lane 2), A549 (lane 3), U87 (lane 4), and Raji (lane 5) using CCR3/CD193 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CCR3/CD193 Antibody (YA7736)
  • CCR3/CD193 Antibody (YA7736) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (40 μg) from HepG2 (lane 2), A549 (lane 3), U87 (lane 4), and Raji (lane 5) using CCR3/CD193 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CCR3/CD193 Antibody (YA7736) Product Image WB