Phospho-Chk1 (Ser317) Antibody (YA7581)

Art. -Nr.: HY-P87896: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-Chk1 (Ser317) Antibody (YA7581) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-Chk1 (Ser317).

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10 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

Phospho-Chk1 (Ser317) Antibody (YA7581) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Phospho-Chk1 (Ser317).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molekulargewicht

Predicted band size: 54 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O14757

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Sensitivity	Endogenous	Reinheit	Protein A affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG, Kappa

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856, PubMed:32357935). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). Recognizes the substrate consensus sequence [R-X-X-S/T] (PubMed:11535615, PubMed:12399544, PubMed:12446774, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15650047, PubMed:15665856). Binds to and phosphorylates CDC25A, CDC25B and CDC25C (PubMed:12676583, PubMed:12676925, PubMed:12759351, PubMed:14559997, PubMed:14681206, PubMed:19734889, PubMed:9278511).

Subcellular Localization：Nucleus,Chromosome,Cytoplasm,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon

Isoforms & Post-Translational Modification：O14757 has three isomers: O14757-1: 54434 Da (predicted); O14757-2: 43703 Da (predicted); O14757-3: 50415 Da (predicted).
Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication (PubMed:10859164, PubMed:11390642, PubMed:12446774, PubMed:12588868, PubMed:12676583, PubMed:12676925, PubMed:12676962, PubMed:14681223, PubMed:14988723, PubMed:15650047, PubMed:15707391, PubMed:15870257, PubMed:25083873, PubMed:31316063)丨Ubiquitinated丨Proteolytically cleaved at the C-terminus by SPRTN during normal DNA replication, thereby promoting CHEK1 removal from chromatin and activating the protein kinase activity

Subunit：Interacts (phosphorylated by ATR) with RAD51 (PubMed:15665856)

Dokumentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/panc-1-cell.html [Content Brief]