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Hsp90 alpha Antibody(YA6866)

(Synonyms: HSP 86; HSP86; LAP-2; LPS-associated protein 2; HSP90AA1)
Customer Review

Based on 1 Customer Validation

Hsp90 alpha Antibody(YA6866) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hsp90 alpha.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Hsp90 alpha Antibody(YA6866) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:500 1:1000 1:1000

Product Details

Description

Hsp90 alpha Antibody(YA6866) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hsp90 alpha.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 85 kDa；
Species Reactivity Database
Immunogen

Synthetic peptide within Human Hsp90 alpha aa 1-50 / 732.

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719279

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Western blot analysis of extracts from HeLa (lane 2(20μg), A549 (lane 3(20μg), 3T3 (lane 4(20μg) and K562 (lane 5(20μg) using Hsp90 alpha Antibody (HY-P87175) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Hsp90 alpha is a Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochondrial outer membrane which promotes host antiviral response |(Microbial infection) Seems to interfere with N.meningitidis NadA-mediated invasion of human cells. Decreasing HSP90 levels increases adhesion and entry of E.coli expressing NadA into human Chang cells; increasing its levels leads to decreased adhesion and invasion[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Nucleus,Cytoplasm,Melanosome,Cell membrane,Mitochondrion

  • Isoforms & Post-Translational Modification

    P07900 has two isomers: P07900-1: 84660 Da (predicted); P07900-2: 98161 Da (predicted).
    ISGylated丨S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1丨Ubiquitinated via 'Lys-63'-linked polyubiquitination by HECTD1

  • Subunit

    Homodimer (PubMed:18400751, PubMed:29127155, PubMed:7588731, PubMed:8289821)

  • SwissProt ID

    P07900

  • Gene ID
    3320 [NCBI]
  • Synonyms

    HSP 86; HSP86; LAP-2; LPS-associated protein 2; HSP90AA1

Hsp90 alpha Antibody(YA6866) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), A549 (lane 3(20μg), 3T3 (lane 4(20μg) and K562 (lane 5(20μg) using Hsp90 alpha Antibody (HY-P87175) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    1/16
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Hsp90 alpha Antibody(YA6866) Product Image
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), A549 (lane 3(20μg), 3T3 (lane 4(20μg) and K562 (lane 5(20μg) using Hsp90 alpha Antibody (HY-P87175) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Experimental Validation Results for Hsp90 alpha Antibody(YA6866)
  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg), A549 (lane 3(20μg), 3T3 (lane 4(20μg) and K562 (lane 5(20μg) using Hsp90 alpha Antibody (HY-P87175) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Hsp90 alpha Antibody (HY-P87175, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp90 alpha Antibody(YA6866) Product Image WB
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P
  • Hsp90 alpha Antibody(YA6866) Product Image IHC-P