PICALM Antibody (YA6703)

Cat. No.: HY-P87010: Data Sheet COA
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PICALM Antibody (YA6703) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PICALM.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PICALM Antibody (YA6703) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PICALM.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 71 kDa;

Observed band size: 65/71 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13492

Gene ID

8301 [NCBI]

Immunogen

Recombinant protein within human PICALM aa 1-450.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PubMed:10436022, PubMed:16262731, PubMed:27574975). Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PubMed:25898166). In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PubMed:21808019, PubMed:22118466, PubMed:23741335). In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PubMed:25241929). Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF) (PubMed:24067654, PubMed:25241929)

Subcellular Localization：Cell membrane,Membrane, clathrin-coated pit,Golgi apparatus,Cytoplasmic vesicle, clathrin-coated vesicle,Nucleus

Expression：
Tissue_Specificity: Expressed in all tissues examined

Isoforms & Post-Translational Modification：Q13492 has 5 isomers: Q13492-1: 70755 Da (predicted); Q13492-2: 68764 Da (predicted); Q13492-3: 66393 Da (predicted); Q13492-4: 59918 Da (predicted); Q13492-5: 70037 Da (predicted).

Subunit：Binds to clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity

Synonyms

CALM antibody; Clathrin assembly lymphoid myeloid leukemia antibody; Clathrin assembly lymphoid myeloid leukemia protein antibody; CLTH antibody; LAP antibody; Phosphatidylinositol-binding clathrin assembly protein antibody; PICAL_HUMAN antibody; PICALM antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)