UCHL3 Antibody (YA2811)

Cat. No.: HY-P83066: Data Sheet COA
SDS
User Guide for Antibodies
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UCHL3 Antibody (YA2811) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL3.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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UCHL3 Antibody (YA2811) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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UCHL3 Antibody (YA2811) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 26 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P15374

Gene ID

7347 [NCBI]

Immunogen

A synthesized peptide derived from human UCHL3 aa1-50/230.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using UCHL3 Antibody (HY-P83066) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Highly expressed in heart, skeletal muscle, and testis

Subunit：Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates

RRID

AB_3105182

Database

Entrez Gene: 7347 Human ; 50933 Mouse ; 498560 Rat

SwissProt: P15374 Human ; Q9JKB1 Mouse ; Q91Y78 Rat

OMIM: 603090 Human

Research Field

Cell Biology

Synonyms

UCH-L3; Ubiquitin thiolesterase L3; Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCHL3

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

UCHL3 Antibody (YA2811) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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UCHL3 Antibody (YA2811)

UCHL3 Antibody (YA2811) Featured Recommendations:

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UCHL3 Antibody (YA2811) Related Classifications

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