Optineurin Antibody (YA6299)
(Synonyms: Optineurin; E3-14.7K-interacting protein; FIP-2; Huntingtin yeast partner L; Huntingtin-interacting protein 7; HIP-7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Transcription factor IIIA-interacting protein; TFIIIA-IntP; )Optineurin Antibody (YA6299) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Optineurin.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-500 | 1:1000-5000 | 1:200-1000 | 1:5000-20000 | 1:50-200 |
Product Details
Optineurin Antibody (YA6299) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Optineurin.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 74 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 63 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Optineurin plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta/IFNB1. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and huntingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TFRC/TfR); regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52; (Microbial infection) May constitute a cellular target for various viruses, such as adenovirus E3 14.7 or Bluetongue virus, to inhibit innate immune response. During RNA virus infection, such as that of Sendai virus, negatively regulates the induction of IFNB1[1][2][3][4][5].
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Subcellular Localization
Cytoplasm, perinuclear region; Golgi apparatus; Golgi apparatus, trans-Golgi network; Cytoplasmic vesicle, autophagosome; Cytoplasmic vesicle; Recycling endosome
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Expression
Tissue_specificity:It is present in the aqueous humor (protein level) . It is expressed in the trabecular meshwork (protein level) (PubMed:11834836, PubMed:12379221, PubMed:12646749) . It is expressed in non-pigmented ciliary body epithelium (protein level) (PubMed:11834836) . It is highly expressed in skeletal muscle and has also been detected in the heart, brain, pancreas, kidney, placenta, and liver (PubMed:9488477) . It is expressed in dermal fibroblasts (protein level) (PubMed:11834836) .
Induction:Up-regulated by TNF (PubMed:10807909, PubMed:12379221, PubMed:9488477) . Up-regulated by IFNG. TNF and IFNG act synergistically to stimulate OPTN expression (PubMed:10807909) . Induced by glucocorticoids, such as dexamethasone (PubMed:12379221) . In an in vitro experimental setting, in which donor eyes are subjected to increased perfusion pressure (10 to 30 mm Hg) in the anterior chamber, there is no up-regulation in the trabecular meshwork at the transcript level for periods ranging between 1 and 24 hours (PubMed:12646749) . However, exposure to continuous elevated pressure for several days shows an induction of OPTN expression, with a 56% increase after 7 days (PubMed:12379221) ; (Microbial infection) Up-regulated in response to Sendai virus infection or double stranded RNA treatment (at protein level) ; the up-regulation is direct and not mediated through a response to type I interferons; this may negatively regulate the interferon response to RNA-activated antiviral signaling pathways -
Isoforms & Post-Translational Modification
Q96CV9 has 3 isomers: Q96CV9-1: 65922 Da (predicted); Q96CV9-2: 65203 Da (predicted); Q96CV9-3: 59560 Da (predicted).
Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection. Phosphorylation is induced by phorbol esters and decreases its half-time -
Subunit
Self-associates (PubMed:23669351). Interacts with HD (PubMed:11137014, PubMed:9700202). Interacts with GTF3A (PubMed:10756201). Interacts with MYO6 (PubMed:31371777). Interacts (via UBAN) with ubiquitinated TFRC (PubMed:20085643). Interacts with GTP-bound Rab8 (RAB8A and/or RAB8B) (PubMed:11137014, PubMed:20085643, PubMed:22854040). Interacts with TBC1D17 (PubMed:22854040). Interacts with TBK1 (PubMed:20174559, PubMed:23669351, PubMed:27538435). Interacts with TRAF3 (PubMed:20174559). Binds to linear ubiquitin chains (PubMed:20085643). Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the association (at least with MAP1LC3B). Interacts with RAB12; the interaction may be indirect. Interacts with TBK1; this interaction leads to the Golgi localization of TBK1 and its subsequent activation. Interacts with palmitoyltransferase ZDHHC17/HIP14; the interaction does not lead to palmitoylation of OPTN (PubMed:24705354). Interacts with CYLD (PubMed:32185393). Interacts with TOM1; the interaction is indirect and is mediated by MYO6, which acts as a bridge between TOM1 and OPTN (PubMed:31371777). Interacts with USP12; the interaction is independent of USP12 deubiquitinase activity and may be involved in regulation of autophagic flux (PubMed:30266909)
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SwissProt ID
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Synonyms
Optineurin; E3-14.7K-interacting protein; FIP-2; Huntingtin yeast partner L; Huntingtin-interacting protein 7; HIP-7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Transcription factor IIIA-interacting protein; TFIIIA-IntP;
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