Copy Link Print Information

ERG Antibody (YA2842)

(Synonyms: ERG; erg-3; p55; KCNH2)
Customer Review

Based on 1 Customer Validation

ERG Antibody (YA2842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERG.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • ERG Antibody (YA2842) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:200-1:1000 1:200-1:1000 1:1000-1:5000

Product Details

Description

ERG Antibody (YA2842) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERG.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ERG aa400-479.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105213

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERG Antibody (YA2842)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Raw264.7 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using ERG Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ERG Antibody (YA2842)
    Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ERG Antibody (YA2842)
    Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ERG transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Isoforms & Post-Translational Modification

    P11308 has 6 isomers: P11308-4: 53838 Da (predicted); P11308-3: 54608 Da (predicted); P11308-1: 52031 Da (predicted); P11308-2: 41397 Da (predicted); P11308-5: 35286 Da (predicted); P11308-6: 36541 Da (predicted).

  • Subunit

    Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SETDB1

  • SwissProt ID

    P11308

  • Gene ID
    2078 [NCBI]
  • Synonyms

    ERG; erg-3; p55; KCNH2

  • Research Field

    Epigenetics and Nuclear Signaling

ERG Antibody (YA2842) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Raw264.7 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using ERG Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ERG Antibody (YA2842) Product Image
    1/16
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • ERG Antibody (YA2842) Product Image
  • Experimental Validation Results for ERG Antibody (YA2842)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Raw264.7 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using ERG Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ERG Antibody (YA2842)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ERG Antibody (YA2842)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • Experimental Validation Results for ERG Antibody (YA2842)
  • ERG Antibody (YA2842) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Raw264.7 (lane 2, 20 μg), Mouse brain (lane 3, 20 μg), and Rat brain (lane 4, 20 μg) using ERG Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ERG Antibody (YA2842) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ERG Antibody (YA2842) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 THP-1 cells labeling ERG Antibody (HY-P83097, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ERG Antibody (YA2842) Product Image WB
  • ERG Antibody (YA2842) Product Image FC
  • ERG Antibody (YA2842) Product Image FC