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Musashi 2 Antibody (YA4422)

(Synonyms: MSI2H; MGC3245; FLJ36569; MSI2)
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Based on 1 Customer Validation

Musashi 2 Antibody (YA4422) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Musashi 2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Musashi 2 Antibody (YA4422)
  • Musashi 2 Antibody (YA4422) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

Musashi 2 Antibody (YA4422) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Musashi 2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Purified recombinant fragment of human MSI2 aa 1-190.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718970

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Musashi 2 Antibody (YA4422)
    Western blot analysis of extracts from K-562 (lane 2(20μg), HeLa (lane 3(20μg), RAji (lane 4(20μg) and HT-29 (lane 5(20μg) using Musashi 2 Antibody (HY-P84725) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Musashi 2 is a RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:It is ubiquitous; it can be detected even at low concentrations.

    Induction:Up-regulated in astrocytes after brain injury

  • Subunit

    Interacts with RHOBTB2; the interaction is necessary for MSI2 ubiquitination and degradation

  • SwissProt ID

    Q96DH6

  • Gene ID
    124540 [NCBI]
  • Synonyms

    MSI2H; MGC3245; FLJ36569; MSI2

Musashi 2 Antibody (YA4422) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K-562 (lane 2(20μg), HeLa (lane 3(20μg), RAji (lane 4(20μg) and HT-29 (lane 5(20μg) using Musashi 2 Antibody (HY-P84725) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Musashi 2 Antibody (YA4422) Product Image
    1/16
  • Experimental Validation Results for Musashi 2 Antibody (YA4422)
  • Musashi 2 Antibody (YA4422) Product Image
  • Experimental Validation Results for Musashi 2 Antibody (YA4422)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K-562 (lane 2(20μg), HeLa (lane 3(20μg), RAji (lane 4(20μg) and HT-29 (lane 5(20μg) using Musashi 2 Antibody (HY-P84725) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Musashi 2 Antibody (YA4422)
  • Musashi 2 Antibody (YA4422) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K-562 (lane 2(20μg), HeLa (lane 3(20μg), RAji (lane 4(20μg) and HT-29 (lane 5(20μg) using Musashi 2 Antibody (HY-P84725) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Musashi 2 Antibody (YA4422) Product Image WB