Musashi 2 Antibody (YA4422)

Cat. No.: HY-P84725: Data Sheet COA
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Musashi 2 Antibody (YA4422) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Musashi 2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Musashi 2 Antibody (YA4422) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Musashi 2.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96DH6

Gene ID

124540 [NCBI]

Immunogen

Purified recombinant fragment of human MSI2 aa 1-190.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K-562 (lane 2(20μg), HeLa (lane 3(20μg), RAji (lane 4(20μg) and HT-29 (lane 5(20μg) using Musashi 2 Antibody (HY-P84725) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is ubiquitous; it can be detected even at low concentrations.

Induction:Up-regulated in astrocytes after brain injury

Subunit：Interacts with RHOBTB2; the interaction is necessary for MSI2 ubiquitination and degradation

RRID

AB_3718970

Synonyms

MSI2H; MGC3245; FLJ36569; MSI2

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)