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LMO4 Antibody (YA2953)

(Synonyms: LIM domain only protein 4; LMO4; )
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Based on 1 Customer Validation

LMO4 Antibody (YA2953) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMO4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • LMO4 Antibody (YA2953) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

LMO4 Antibody (YA2953) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMO4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 18 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human LMO4

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105323

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LMO4 Antibody (YA2953)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and MCF-7 (lane 5, 15 μg) using LMO4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for LMO4 Antibody (YA2953)
    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using LMO4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    LMO4 transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons

  • Subunit

    Interacts strongly with LDBS. Interacts with LDB2 and LDB1.

  • SwissProt ID

    P61968

  • Gene ID
    8543 [NCBI]
  • Synonyms

    LIM domain only protein 4; LMO4;

  • Research Field

    Epigenetics and Nuclear Signaling

LMO4 Antibody (YA2953) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and MCF-7 (lane 5, 15 μg) using LMO4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using LMO4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LMO4 Antibody (YA2953) Product Image
    1/16
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • LMO4 Antibody (YA2953) Product Image
  • Experimental Validation Results for LMO4 Antibody (YA2953)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and MCF-7 (lane 5, 15 μg) using LMO4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for LMO4 Antibody (YA2953)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using LMO4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • Experimental Validation Results for LMO4 Antibody (YA2953)
  • LMO4 Antibody (YA2953) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and MCF-7 (lane 5, 15 μg) using LMO4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • LMO4 Antibody (YA2953) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using LMO4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • LMO4 Antibody (YA2953) Product Image WB
  • LMO4 Antibody (YA2953) Product Image WB