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  4. LMO4 Antibody (YA2953)

LMO4 Antibody (YA2953) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMO4.

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容量 価格(税別) 在庫状況 数量
無料サンプル   今すぐ申し込む  
10 μL $84 在庫あり
50 μL $222 在庫あり
100 μL $360 在庫あり
250 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

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  • 説明

製品説明

LMO4 Antibody (YA2953) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LMO4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量
Predicted band size: 18 kDa;
Observed band size: 18 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human LMO4

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous 純度 Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Jurkat (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), 3T3 (lane 4, 15 μg), and MCF-7 (lane 5, 15 μg) using LMO4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), NIH/3T3 (lane 4(20μg), using LMO4 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background
Function:Transcription cofactor. Plays a role in establishing motor neuron identity, in concert with MNX1, acting, at least in part, to disrupt LDB1-LHX3 complexes thereby negatively modulating interneuron genes in motor neurons
Subunit:Interacts strongly with LDBS. Interacts with LDB2 and LDB1.
RRID
Database
Research Field

Epigenetics and Nuclear Signaling

Synonyms
LIM domain only protein 4; LMO4;
ドキュメンテーション
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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備考

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Inquiry Information

製品名:
LMO4 Antibody (YA2953)
製品番号:
HY-P83208
数量:
MCE 日本正規代理店: