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RAP1A+RAP1B Antibody (YA1824)

(Synonyms: Ras-related protein Rap-1b; K-REV; RAL1B)
Customer Review

Based on 1 Customer Validation

RAP1A+RAP1B Antibody (YA1824) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1A+RAP1B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • RAP1A+RAP1B Antibody (YA1824) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:20-1:50 1:20-1:50

Product Details

Description

RAP1A+RAP1B Antibody (YA1824) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAP1A+RAP1B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human RAP1A/B

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104198

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using RAP1A+RAP1B Antibody (HY-P82079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), HUVEC (lane 3, 15 μg), HT-1080 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using RAP1A + RAP1B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    Flow cytometric analysis of 1X106 HeLa cells labeling RAP1A+RAP1B Antibody (HY-P82079, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    RAP1A+RAP1B is a GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function

  • Subcellular Localization

    Cell membrane; Cytoplasm, cytosol; Cell junction

  • Isoforms & Post-Translational Modification

    P61224 has 4 isomers: P61224-1: 20825 Da (predicted); P61224-2: 15356 Da (predicted); P61224-3: 18778 Da (predicted); P61224-4: 16033 Da (predicted).

  • Subunit

    Heterodimer with RAP1GAP (PubMed:18309292). Interacts with EPAC2 (PubMed:18660803). Interacts with SGSM1 (PubMed:17509819). Interacts with SGSM2 (PubMed:17509819). Interacts with SGSM3 (PubMed:17509819). Interacts with KRIT1 (PubMed:22577140). Interacts with RAP1GDS1 (PubMed:24415755)

  • SwissProt ID

    P61224

  • Gene ID
    5908 [NCBI]
  • Synonyms

    Ras-related protein Rap-1b; K-REV; RAL1B

  • Research Field

    Signal Transduction

RAP1A+RAP1B Antibody (YA1824) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using RAP1A+RAP1B Antibody (HY-P82079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), HUVEC (lane 3, 15 μg), HT-1080 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using RAP1A + RAP1B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling RAP1A+RAP1B Antibody (HY-P82079, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • RAP1A+RAP1B Antibody (YA1824) Product Image
    1/16
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • RAP1A+RAP1B Antibody (YA1824) Product Image
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using RAP1A+RAP1B Antibody (HY-P82079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), HUVEC (lane 3, 15 μg), HT-1080 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using RAP1A + RAP1B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling RAP1A+RAP1B Antibody (HY-P82079, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • Experimental Validation Results for RAP1A+RAP1B Antibody (YA1824)
  • RAP1A+RAP1B Antibody (YA1824) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using RAP1A+RAP1B Antibody (HY-P82079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RAP1A+RAP1B Antibody (YA1824) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), HUVEC (lane 3, 15 μg), HT-1080 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using RAP1A + RAP1B Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • RAP1A+RAP1B Antibody (YA1824) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling RAP1A+RAP1B Antibody (HY-P82079, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • RAP1A+RAP1B Antibody (YA1824) Product Image WB
  • RAP1A+RAP1B Antibody (YA1824) Product Image WB
  • RAP1A+RAP1B Antibody (YA1824) Product Image FC