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Vasopressin V1a Receptor Antibody

(Synonyms: AVPR1, AVPR1A, Vasopressin V1a receptor, V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor)
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Based on 1 Customer Validation

Vasopressin V1a Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Vasopressin V1a Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Vasopressin V1a Receptor Antibody
  • Vasopressin V1a Receptor Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1000 1:50-200

Product Details

Description

Vasopressin V1a Receptor Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Vasopressin V1a Receptor.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Purified recombinant protein of human Vasopressin V1a Receptor

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vasopressin V1a Receptor Antibody
    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), MCF-7 (lane 3) and K562 (lane 4) using Vasopressin V1a Receptor antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Vasopressin V1a Receptor is a Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Has been involved in social behaviors, including affiliation and attachment

  • Subcellular Localization

    Cell membrane

  • Isoforms & Post-Translational Modification

    Vasopressin V1a Receptor has an amino acid length of 418, molecular weight is 46800 Da.

  • SwissProt ID

    P37288

  • Gene ID
    552 [NCBI]
  • Synonyms

    AVPR1, AVPR1A, Vasopressin V1a receptor, V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor

Vasopressin V1a Receptor Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), MCF-7 (lane 3) and K562 (lane 4) using Vasopressin V1a Receptor antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Vasopressin V1a Receptor Antibody Product Image
    1/16
  • Experimental Validation Results for Vasopressin V1a Receptor Antibody
  • Vasopressin V1a Receptor Antibody Product Image
  • Experimental Validation Results for Vasopressin V1a Receptor Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), MCF-7 (lane 3) and K562 (lane 4) using Vasopressin V1a Receptor antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Vasopressin V1a Receptor Antibody
  • Vasopressin V1a Receptor Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from A549 (lane 2), MCF-7 (lane 3) and K562 (lane 4) using Vasopressin V1a Receptor antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Vasopressin V1a Receptor Antibody Product Image WB