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Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)

(Synonyms: G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD)
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Based on 1 Customer Validation

Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:50-200 1:50-200 1:50-100

Product Details

Description

Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Immunogen

Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 460-509 / 515.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)
    Western blot analysis of extracts from A549 (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SW480 (lane 5, 20ug) using Glucose 6 Phosphate Dehydrogenase Antibody (HY-P810629). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Glucose 6 Phosphate Dehydrogenase catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Also catalyzes the conversion of NAADPH, which is produced by enzymes such as DUOX1, DUOX2 and NOX5 from NAADP and promotes Ca(2+) signaling during T cell activation, back to NAADP[1].

  • Subcellular Localization

    Cytoplasm, cytosol; Membrane

  • Expression


    Tissue_Specificity: Isoform Long is found in lymphoblasts, granulocytes and sperm.

  • Isoforms & Post-Translational Modification

    Glucose 6 Phosphate Dehydrogenase has 3 isoforms, P11413-1: amino acid length is 515, molecular weight is 59257 Da (predicted); P11413-2: amino acid length is 561, molecular weight is 63827 Da (predicted); P11413-3: amino acid length is 545, molecular weight is 62468 Da (predicted).Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity

  • Subunit

    Homotetramer; dimer of dimers.

  • SwissProt ID

    P11413

  • Gene ID
    2539 [NCBI]
  • Synonyms

    G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD

References

[1]. Gu F, et al. Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca(2+) signaling during T cell activation. Sci Signal. 2021 Nov 16;14(709):eabe3800. [Content Brief]

Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SW480 (lane 5, 20ug) using Glucose 6 Phosphate Dehydrogenase Antibody (HY-P810629). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Product Image
    1/16
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Product Image
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SW480 (lane 5, 20ug) using Glucose 6 Phosphate Dehydrogenase Antibody (HY-P810629). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucose 6 Phosphate Dehydrogenase Antibody (YA9891)
  • Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SW480 (lane 5, 20ug) using Glucose 6 Phosphate Dehydrogenase Antibody (HY-P810629). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Glucose 6 Phosphate Dehydrogenase Antibody (YA9891) Product Image WB