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alpha Skeletal Muscle Actin Antibody (YA930)

(Synonyms: ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1)
Customer Review

Based on 1 Customer Validation

alpha Skeletal Muscle Actin Antibody (YA930) is a mouse-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA930), targeting alpha Skeletal Muscle Actin. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

alpha Skeletal Muscle Actin Antibody (YA930) is a mouse-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA930), targeting alpha Skeletal Muscle Actin. It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic Peptide of α skeletal muscle actin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103305

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    Western blot analysis of extracts from Hela (lane 2(20μg), A431(lane 3(20μg) and NIH3T3 (lane 4(20μg) using alpha Skeletal Muscle Actin (HY-P81258) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    Immunocytochemistry analysis of HELA cells labeling alpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    Immunocytochemistry analysis of HELA cells labelingalpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    alpha Skeletal Muscle Actin is an Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Subunit

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with alpha-actinin (By similarity). Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity). Interacts with TTID (PubMed:10958653). Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells (PubMed:16501887)

  • SwissProt ID

    P68133

  • Gene ID
    58 [NCBI]
  • Synonyms

    ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1

  • Research Field

    Signal Transduction

alpha Skeletal Muscle Actin Antibody (YA930) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), A431(lane 3(20μg) and NIH3T3 (lane 4(20μg) using alpha Skeletal Muscle Actin (HY-P81258) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labeling alpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labelingalpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    1/16
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), A431(lane 3(20μg) and NIH3T3 (lane 4(20μg) using alpha Skeletal Muscle Actin (HY-P81258) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labeling alpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HELA cells labelingalpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • Experimental Validation Results for alpha Skeletal Muscle Actin Antibody (YA930)
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), A431(lane 3(20μg) and NIH3T3 (lane 4(20μg) using alpha Skeletal Muscle Actin (HY-P81258) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HELA cells labeling alpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HELA cells labelingalpha Skeletal Muscle Actin with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with alpha Skeletal Muscle Actin Antibody (HY-P81258) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using alpha Skeletal Muscle Actin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image WB
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image ICC
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image ICC
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image IHC-P
  • alpha Skeletal Muscle Actin Antibody (YA930) Product Image IHC-P