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Frizzled 8 Antibody (YA2052)

(Synonyms: Frizzled-8; FZ8; FZD8; hFz8)
Customer Review

Based on 1 Customer Validation

Frizzled 8 Antibody (YA2052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Frizzled 8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Frizzled 8 Antibody (YA2052) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Frizzled 8 Antibody (YA2052) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Frizzled 8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 73 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 73 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Frizzled 8 aa640-694/694.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104418

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using Frizzled 8 Antibody (HY-P82307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human breast cancera tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    Frizzled 8 receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1

  • Subcellular Localization

    Membrane; Multi-pass membrane protein; Golgi apparatus; Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Most abundant in fetal kidney, followed by brain and lung. In human tissues, expressed in kidney, heart, pancreas and skeletal muscle

  • Subunit

    Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly with LRP5 or LRP6; the interaction is promoted by Wnt-binding and signaling and inhibited by DKK1. Interacts with GPOC, RSPO1 and RSPO3 (By similarity). Interacts with glypican GPC3 (PubMed:24496449)

  • SwissProt ID

    Q9H461

  • Gene ID
    8325 [NCBI]
  • Synonyms

    Frizzled-8; FZ8; FZD8; hFz8

  • Research Field

    Signal Transduction

Frizzled 8 Antibody (YA2052) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using Frizzled 8 Antibody (HY-P82307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancera tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    1/16
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Frizzled 8 Antibody (YA2052) Product Image
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using Frizzled 8 Antibody (HY-P82307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancera tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Experimental Validation Results for Frizzled 8 Antibody (YA2052)
  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using Frizzled 8 Antibody (HY-P82307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancera tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix of uterus tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue using Frizzled 8 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82307, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Frizzled 8 Antibody (YA2052) Product Image WB
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P
  • Frizzled 8 Antibody (YA2052) Product Image IHC-P