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KPNB1 Antibody (YA6627)

(Synonyms: IMB 1 antibody; IMB1 antibody; IMB1_HUMAN antibody; Impnb antibody; Importin 90 antibody; Importin beta 1 antibody; Importin beta 1 subunit antibody; Importin subunit beta-1 antibody; Importin-90 antibody; IPOB antibody; IMB 1 antibody; IMB1 antibody; IMB1_HUMAN antibody; Impnb antibody; Importin 90 antibody; Importin beta 1 antibody; Importin beta 1 subunit antibody; Importin subunit beta-1 antibody; Importin-90 antibody; IPOB antibody; Karyopherin beta 1 antibody; Karyopherin beta 1 subunit antibody; Karyopherin subunit beta-1 antibody; KPNB 1 antibody; Kpnb1 antibody; MGC2155 antibody; MGC2156 antibody; MGC2157 antibody; NTF 97 antibody; NTF97 antibody; NTF97/Importin beta antibody; Nuclear factor P97 antibody; Pore targeting complex 97 kDa subunit antibody; PTAC97 antibody; )
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Based on 1 Customer Validation

KPNB1 Antibody (YA6627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KPNB1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KPNB1 Antibody (YA6627)
  • KPNB1 Antibody (YA6627) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:1000 1:200 1-2μg/sample

Product Details

Description

KPNB1 Antibody (YA6627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KPNB1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 97 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97.2 kDa
Immunogen

Recombinant protein within human KPNB1 aa 1-550.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719230

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KPNB1 Antibody (YA6627)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using KPNB1 Antibody (HY-P86934) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    KPNB1 functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports MRTFA, SNAI1 and PRKCI into the nucleus |(Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cytoplasm,Nucleus envelope

  • Isoforms & Post-Translational Modification

    Q14974 has two isomers: Q14974-1: 97170 Da (predicted); Q14974-2: 81179 Da (predicted).
    Mono-ADP-ribosylated by PARP16

  • Subunit

    Forms a complex with an importin alpha subunit (PubMed:20818336, PubMed:8617227, PubMed:8692944)

  • SwissProt ID

    Q14974

  • Gene ID
    3837 [NCBI]
  • Synonyms

    IMB 1 antibody; IMB1 antibody; IMB1_HUMAN antibody; Impnb antibody; Importin 90 antibody; Importin beta 1 antibody; Importin beta 1 subunit antibody; Importin subunit beta-1 antibody; Importin-90 antibody; IPOB antibody; IMB 1 antibody; IMB1 antibody; IMB1_HUMAN antibody; Impnb antibody; Importin 90 antibody; Importin beta 1 antibody; Importin beta 1 subunit antibody; Importin subunit beta-1 antibody; Importin-90 antibody; IPOB antibody; Karyopherin beta 1 antibody; Karyopherin beta 1 subunit antibody; Karyopherin subunit beta-1 antibody; KPNB 1 antibody; Kpnb1 antibody; MGC2155 antibody; MGC2156 antibody; MGC2157 antibody; NTF 97 antibody; NTF97 antibody; NTF97/Importin beta antibody; Nuclear factor P97 antibody; Pore targeting complex 97 kDa subunit antibody; PTAC97 antibody;

KPNB1 Antibody (YA6627) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using KPNB1 Antibody (HY-P86934) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • KPNB1 Antibody (YA6627) Product Image
    1/16
  • Experimental Validation Results for KPNB1 Antibody (YA6627)
  • KPNB1 Antibody (YA6627) Product Image
  • Experimental Validation Results for KPNB1 Antibody (YA6627)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using KPNB1 Antibody (HY-P86934) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KPNB1 Antibody (YA6627)
  • KPNB1 Antibody (YA6627) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using KPNB1 Antibody (HY-P86934) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • KPNB1 Antibody (YA6627) Product Image WB