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ME2 Antibody (YA3055)(PBS only)

(Synonyms: ODS1)

ME2 Antibody (YA3055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ME2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ME2 Antibody (YA3055)(PBS only)
  • ME2 Antibody (YA3055)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

ME2 Antibody (YA3055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ME2.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Immunogen

A synthetic peptide of human ME2

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ME2 Antibody (YA3055)(PBS only)
    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), LnCap (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and K562 (lane 6, 15 μg) using ME2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    ME2 is a NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    P23368 has 2 isomers: P23368-1: 65444 Da (predicted); P23368-2: 53586 Da (predicted).

  • Subunit

    Homotetramer

  • SwissProt ID

    P23368

  • Gene ID
    4200 [NCBI]
  • Synonyms

    ODS1

  • Research Field

    Signal Transduction

ME2 Antibody (YA3055)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), LnCap (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and K562 (lane 6, 15 μg) using ME2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ME2 Antibody (YA3055)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ME2 Antibody (YA3055)(PBS only)
  • ME2 Antibody (YA3055)(PBS only) Product Image
  • Experimental Validation Results for ME2 Antibody (YA3055)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), LnCap (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and K562 (lane 6, 15 μg) using ME2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ME2 Antibody (YA3055)(PBS only)
  • ME2 Antibody (YA3055)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HL-60 (lane 1, 15 μg), Hela (lane 2, 15 μg), Jurkat (lane 3, 15 μg), LnCap (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and K562 (lane 6, 15 μg) using ME2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ME2 Antibody (YA3055)(PBS only) Product Image WB