VAP-B/C Antibody (YA9088)
(Synonyms: UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C)
Based on 1 Customer Validation
VAP-B/C Antibody (YA9088) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VAP-B/C.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
VAP-B/C Antibody (YA9088) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to VAP-B/C.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 27 kDa
OMIM: 608627
A synthesized peptide derived from human VAP-B/C.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
VAP-B/C is an Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation[1][2][3][4][5].
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Subcellular Localization
Endoplasmic reticulum membrane
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Expression
Tissue_Specificity: Ubiquitous. Isoform 1 predominates. -
Isoforms & Post-Translational Modification
VAP-B/C has 2 isoforms, O95292-1: amino acid length is 243, molecular weight is 27228 Da (predicted); O95292-2: amino acid length is 99, molecular weight is 11152 Da (predicted).
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Subunit
Homodimer, and heterodimer with VAPA.
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SwissProt ID
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Synonyms
UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C
Documentation
References
[1]. Lucken-Ardjomande Häsler S, et al. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508. J Cell Biol. 2020 May 4;219(5):. [Content Brief]
[2]. Di Mattia T, et al. FFAT motif phosphorylation controls formation and lipid transfer function of inter-organelle contacts. EMBO J. 2020 Dec 1;39(23):e104369. [Content Brief]
[3]. Kanekura K, et al. Characterization of amyotrophic lateral sclerosis-linked P56S mutation of vesicle-associated membrane protein-associated protein B (VAPB/ALS8). J Biol Chem. 2006 Oct 6;281(40):30223-33. [Content Brief]
[4]. Chen HJ, et al. Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis. J Biol Chem. 2010 Dec 17;285(51):40266-81. [Content Brief]
[5]. De Vos KJ, et al. VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis. Hum Mol Genet. 2012 Mar 15;21(6):1299-311. [Content Brief]
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