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TCF-4/TCF7L2 Antibody (YA039)

(Synonyms: TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4)
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Based on 1 publication(s) in Google Scholar

TCF-4/TCF7L2 Antibody (YA039) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • TCF-4/TCF7L2 Antibody (YA039) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

TCF-4/TCF7L2 Antibody (YA039) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa；
Species Reactivity Database

Entrez Gene: 6934 Human

SwissProt: Q9NQB0 Human

Immunogen

Synthetic peptide corresponding to Human TCF7L2.AA range:1-49.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102117

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), Jurkat (lane4(20μg) and mouse testis tissue (lane5(20μg) using TCF-4/TCF7L2 Antibody (HY-P80520). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Flow cytometric analysis of 1X106 Jurkat cells labeling TCF-4/TCF7L2 Antibody (HY-P80520, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TCF-4/TCF7L2 participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine

  • Subcellular Localization

    Nucleus, PML body; Nucleus

  • Expression


    Tissue_specificity:This gene was detected in small intestinal epithelial cells, with the highest expression at the top of the crypts and a gradient of expression from the crypts to the villi. It was also detected in colonic epithelial cells and colon cancer, as well as in breast epithelial cells and their derivative carcinomas.

  • Subunit

    Interacts with TGFB1I1 (By similarity). Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex (PubMed:10080941, PubMed:17052462, PubMed:19816403, PubMed:28829046, PubMed:29739711, PubMed:9065401, PubMed:9916915). Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Interacts with DDIT3/CHOP. The CTNNB1 and TCF7L2/TCF4 complex interacts with PML (isoform PML-4). Identified in a complex with CTNNB1 and FERMT2. Interacts with SPIN1 (PubMed:22258766, PubMed:24589551, PubMed:29061846). Interacts with C11orf84/SPINDOC in a SPIN1-dependent manner (PubMed:29061846). Interacts with DAZAP2; the interaction results in localization of DAZAP2 to the nucleus (PubMed:19304756)

  • SwissProt ID

    Q9NQB0

  • Gene ID
    6934 [NCBI]
  • Synonyms

    TCF4, TCF7L2, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4

  • Research Field

    Epigenetics and Nuclear Signaling

TCF-4/TCF7L2 Antibody (YA039) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), Jurkat (lane4(20μg) and mouse testis tissue (lane5(20μg) using TCF-4/TCF7L2 Antibody (HY-P80520). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling TCF-4/TCF7L2 Antibody (HY-P80520, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    1/16
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • TCF-4/TCF7L2 Antibody (YA039) Product Image
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), Jurkat (lane4(20μg) and mouse testis tissue (lane5(20μg) using TCF-4/TCF7L2 Antibody (HY-P80520). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling TCF-4/TCF7L2 Antibody (HY-P80520, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA039)
  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), HepG2 (lane3(20μg), Jurkat (lane4(20μg) and mouse testis tissue (lane5(20μg) using TCF-4/TCF7L2 Antibody (HY-P80520). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue using TCF7L2/TCF4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80520, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling TCF-4/TCF7L2 Antibody (HY-P80520, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCF-4/TCF7L2 Antibody (YA039) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TCF7L2 with TCF7L2 Antibody (HY-P80520) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TCF7L2 Antibody (HY-P80520) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCF-4/TCF7L2 Antibody (YA039) Product Image WB
  • TCF-4/TCF7L2 Antibody (YA039) Product Image IHC-P
  • TCF-4/TCF7L2 Antibody (YA039) Product Image IHC-P
  • TCF-4/TCF7L2 Antibody (YA039) Product Image FC
  • TCF-4/TCF7L2 Antibody (YA039) Product Image ICC
  • TCF-4/TCF7L2 Antibody (YA039) Product Image ICC