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PAX7 Antibody (YA3152)

(Synonyms: Paired box protein Pax-7; HuP1; PAX7; HUP1)
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Based on 1 Customer Validation

PAX7 Antibody (YA3152) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PAX7 Antibody (YA3152)
  • PAX7 Antibody (YA3152) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PAX7 Antibody (YA3152) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX7.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PAX7 aa384-441.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105518

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAX7 Antibody (YA3152)
    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using PAX7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PAX7 transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P23759 has 3 isomers: P23759-3: 55119 Da (predicted); P23759-1: 56896 Da (predicted); P23759-2: 56726 Da (predicted).
    Acetylation at Lys-105 and Lys-139 by KAT8 is required for high-level transcription factor activity. Deacetylated by SIRT2

  • Subunit

    Can bind to DNA as a heterodimer with PAX3. Interacts with PAXBP1; the interaction links PAX7 to a WDR5-containing histone methyltransferase complex (By similarity). Interacts with DAXX (PubMed:10393185)

  • SwissProt ID

    P23759

  • Gene ID
    5081 [NCBI]
  • Synonyms

    Paired box protein Pax-7; HuP1; PAX7; HUP1

  • Research Field

    Epigenetics and Nuclear Signaling

PAX7 Antibody (YA3152) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using PAX7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PAX7 Antibody (YA3152) Product Image
    1/16
  • Experimental Validation Results for PAX7 Antibody (YA3152)
  • PAX7 Antibody (YA3152) Product Image
  • Experimental Validation Results for PAX7 Antibody (YA3152)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using PAX7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PAX7 Antibody (YA3152)
  • PAX7 Antibody (YA3152) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using PAX7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PAX7 Antibody (YA3152) Product Image WB