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CBX4 Antibody (YA2490)

(Synonyms: CBX4; hPc2; NBP16; PC2)
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Based on 1 Customer Validation

CBX4 Antibody (YA2490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CBX4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CBX4 Antibody (YA2490)
  • CBX4 Antibody (YA2490) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

CBX4 Antibody (YA2490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CBX4.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CBX4 aa500-560/560.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CBX4 Antibody (YA2490)
    Western blot analysis was performed on extracts from Raw264.7 (lane 1, 20 μg), C6 (lane 2, 20 μg), 293 (lane 3, 20 μg), PC-3 (lane 4, 20 μg), and Ramos (lane 5, 20 μg) using CBX4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    CBX4 is an E3 SUMO-protein ligase that catalyzes sumoylation of target proteins by promoting the transfer of SUMO from the E2 enzyme to the substrate. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131; Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3). Plays a role in the lineage differentiation of the germ layers in embryonic development[1][2][3][4][5].

  • Subcellular Localization

    Nucleus; Nucleus speckle

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    O00257 has 2 isomers: O00257-1: 61368 Da (predicted); O00257-3: 31987 Da (predicted).
    Ubiquitinated. Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex. Deubiquitinated by USP26;Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK

  • Subunit

    Interacts with histone H3-K9Me3 (By similarity). Component of a PRC1-like complex (PubMed:12167701, PubMed:19636380, PubMed:21282530)

  • SwissProt ID

    O00257

  • Gene ID
    8535 [NCBI]
  • Synonyms

    CBX4; hPc2; NBP16; PC2

  • Research Field

    Epigenetics and Nuclear Signaling

CBX4 Antibody (YA2490) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raw264.7 (lane 1, 20 μg), C6 (lane 2, 20 μg), 293 (lane 3, 20 μg), PC-3 (lane 4, 20 μg), and Ramos (lane 5, 20 μg) using CBX4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CBX4 Antibody (YA2490) Product Image
    1/16
  • Experimental Validation Results for CBX4 Antibody (YA2490)
  • CBX4 Antibody (YA2490) Product Image
  • Experimental Validation Results for CBX4 Antibody (YA2490)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raw264.7 (lane 1, 20 μg), C6 (lane 2, 20 μg), 293 (lane 3, 20 μg), PC-3 (lane 4, 20 μg), and Ramos (lane 5, 20 μg) using CBX4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CBX4 Antibody (YA2490)
  • CBX4 Antibody (YA2490) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Raw264.7 (lane 1, 20 μg), C6 (lane 2, 20 μg), 293 (lane 3, 20 μg), PC-3 (lane 4, 20 μg), and Ramos (lane 5, 20 μg) using CBX4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CBX4 Antibody (YA2490) Product Image WB