ATP6V1A Antibody (YA2211)(PBS only)

Cat. No.: HY-P82466A: Data Sheet User Guide for Antibodies
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ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.

Host

Rabbit

Molecular Weight

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P38606

Gene ID

523 [NCBI]

Immunogen

Recombinant protein of human ATP6V1A aa62-78.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857); (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction

Subcellular Localization：Cytoplasm; Cytoplasm, cytosol; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Lysosome

Expression：
Tissue_specificity:High expression in the skin

Isoforms & Post-Translational Modification：P38606 has 2 isomers: P38606-1: 68304 Da (predicted); P38606-2: 64736 Da (predicted).
Phosphorylation at Ser-384 by AMPK down-regulates its enzyme activity

Subunit：V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:33065002).

Research Field

Tags & Cell Markers

Synonyms

HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hepg2-cell.html [Content Brief]