ATP6V1A Antibody (YA2211)(PBS only)
(Synonyms: HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1)ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 68 kDa
Recombinant protein of human ATP6V1A aa62-78.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg),3T3 (lane 4(20μg), using ATP6V1A Antibody (YA2211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
ATP6V1A is a Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity; (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cytoplasm, cytosol; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Lysosome
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Expression
Tissue_specificity:High expression in the skin -
Isoforms & Post-Translational Modification
P38606 has 2 isomers: P38606-1: 68304 Da (predicted); P38606-2: 64736 Da (predicted).
Phosphorylation at Ser-384 by AMPK down-regulates its enzyme activity -
Subunit
V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:33065002).
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SwissProt ID
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Synonyms
HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1
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Research Field
Tags & Cell Markers
Documentation
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