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ACSL3 Antibody (YA6455)

(Synonyms: ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; Long chain fatty acid CoA ligase 3 antibody; Long-chain acyl-CoA synthetase 3 antibody; Long-chain-fatty-acid--CoA ligase 3 antibody; PRO2194 antibody; )
Customer Review

Based on 1 Customer Validation

ACSL3 Antibody (YA6455) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSL3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • ACSL3 Antibody (YA6455) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:500

Product Details

Description

ACSL3 Antibody (YA6455) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSL3.

  • Host Mouse
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 80 kDa
Immunogen

Recombinant protein within human ACSL3 aa 180-380.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、A431 (lane 3(20μg)) and LNCaP (lane 4(20μg)) using ACSL3 Antibody (HY-P86762) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human skin tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human soft tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    ACSL3 is an Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity[1][2].

  • Subcellular Localization

    Mitochondrion outer membrane,Peroxisome membrane,Microsome membrane,Endoplasmic reticulum membrane

  • Isoforms & Post-Translational Modification

    O95573: 720 amino acids, molecular weight 80420 Da.

  • SwissProt ID

    O95573

  • Gene ID
    2181 [NCBI]
  • Synonyms

    ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; Long chain fatty acid CoA ligase 3 antibody; Long-chain acyl-CoA synthetase 3 antibody; Long-chain-fatty-acid--CoA ligase 3 antibody; PRO2194 antibody;

ACSL3 Antibody (YA6455) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、A431 (lane 3(20μg)) and LNCaP (lane 4(20μg)) using ACSL3 Antibody (HY-P86762) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    1/16
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • ACSL3 Antibody (YA6455) Product Image
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、A431 (lane 3(20μg)) and LNCaP (lane 4(20μg)) using ACSL3 Antibody (HY-P86762) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human soft tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • Experimental Validation Results for ACSL3 Antibody (YA6455)
  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、A431 (lane 3(20μg)) and LNCaP (lane 4(20μg)) using ACSL3 Antibody (HY-P86762) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skin tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human soft tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart muscle tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ACSL3 Antibody (HY-P86762, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ACSL3 Antibody (YA6455) Product Image WB
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P
  • ACSL3 Antibody (YA6455) Product Image IHC-P