ACSL3 Antibody (YA6455)

Cat. No.: HY-P86762: Data Sheet COA
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ACSL3 Antibody (YA6455) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSL3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

ACSL3 Antibody (YA6455) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSL3.

Host

Mouse

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 80 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95573

Gene ID

2181 [NCBI]

Immunogen

Recombinant protein within human ACSL3 aa 180-380.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、A431 (lane 3(20μg)) and LNCaP (lane 4(20μg)) using ACSL3 Antibody (HY-P86762) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:22633490). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (PubMed:18003621). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity (By similarity)

Subcellular Localization：Mitochondrion outer membrane,Peroxisome membrane,Microsome membrane,Endoplasmic reticulum membrane

Isoforms & Post-Translational Modification：O95573: 720 amino acids, molecular weight 80420 Da.

Synonyms

ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; ACS3 antibody; Acsl3 antibody; ACSL3_HUMAN antibody; Acyl CoA synthetase long chain family member 3 antibody; FACL3 antibody; Fatty acid Coenzyme A ligase long chain 3 antibody; LACS 3 antibody; LACS3 antibody; Lignoceroyl CoA synthase antibody; Long chain acyl CoA synthetase 3 antibody; Long chain fatty acid CoA ligase 3 antibody; Long-chain acyl-CoA synthetase 3 antibody; Long-chain-fatty-acid--CoA ligase 3 antibody; PRO2194 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)