GPR18 Antibody
(Synonyms: GPCRW, GPR18, N-arachidonyl glycine receptor, NAGly receptor, G-protein coupled receptor 18)GPR18 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GPR18.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 | 1:100-400 |
Product Details
GPR18 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to GPR18.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 38 kDa
Synthetic peptide corresponding to the center region of human GPR18.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GPR18 is a G protein-coupled receptor (GPCR) that plays a role in diverse physiological processes particularly within the immune and nervous systems. Becomes active when triggered by various endogenous ligands including endocannabinoid N-arachidonyl glycine (NAGly), delta-9-tetrahydrocannabinol or resolvin D2/RvD2 derived from the omega-3 fatty acid docosahexaenoic acid (DHA). Upon RvD2 binding, facilitates the resolution of inflammation, aiding in tissue repair and homeostasis. Mechanistically, RvD2 ligation initiates Galphas protein coupling, activation of cAMP-PKA signaling pathway and phosphorylation of STAT3, leading to RvD2-stimulated macrophage phagocytosis. Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis. Activation by N-arachidonoyl glycine (NAGly) can also induce apoptosis in macrophages (By similarity). Plays a role in homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs) in small intestine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Also participates in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane; Cytoplasmic vesicle membrane
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Expression
Tissue_Specificity: Expressed in midpiece of spermatozoon (at protein level). Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells. -
Isoforms & Post-Translational Modification
GPR18 has an amino acid length of 331, molecular weight is 38134 Da.
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SwissProt ID
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Synonyms
GPCRW, GPR18, N-arachidonyl glycine receptor, NAGly receptor, G-protein coupled receptor 18
Documentation
[1]. Burstein SH, et al. Resolution of inflammation by N-arachidonoylglycine. J Cell Biochem. 2011 Nov;112(11):3227-33. [Content Brief]
[2]. Chiang N, et al. Identification of resolvin D2 receptor mediating resolution of infections and organ protection. J Exp Med. 2015 Jul 27;212(8):1203-17. [Content Brief]
[3]. Kohno M, et al. Identification of N-arachidonylglycine as the endogenous ligand for orphan G-protein-coupled receptor GPR18. Biochem Biophys Res Commun. 2006 Sep 1;347(3):827-32. [Content Brief]
[4]. Console-Bram L, et al. Activation of GPR18 by cannabinoid compounds: a tale of biased agonism. Br J Pharmacol. 2014 Aug;171(16):3908-17. [Content Brief]
[5]. Flegel C, et al. Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18. Sci Rep. 2016 Aug 30;6:32255. [Content Brief]
[6]. Chiang N, et al. Novel Resolvin D2 Receptor Axis in Infectious Inflammation. J Immunol. 2017 Jan 15;198(2):842-851. [Content Brief]
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