Pyrin Antibody (YA6885)
(Synonyms: FMF antibody)Pyrin Antibody (YA6885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyrin.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:1000 | 1:1000 |
Product Details
Pyrin Antibody (YA6885) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyrin.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 86 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size：86 kDa
Recombinant protein within Human Pyrin aa 450-781.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Pyrin is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production. Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection. It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cytoplasm, cytoskeleton,Cell projection, ruffle,Cell projection, lamellipodium,Nucleus,Cytoplasm,Cytoplasmic vesicle, autophagosome,Nucleus
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Expression
Tissue_Specificity: Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines
Induction: In monocytes, up-regulated by treatment with colchicine and IFN-alpha, by the pro-inflammatory cytokines IFNG/IFN-gamma and TNF, by bacterial lipopolysaccharides (LPS) and by retroviral infection. Repressed in monocytes by the anti-inflammatory cytokines IL10/interleukin-10, TGFB1 and IL4/interleukin-4. In neutrophils and macrophages, up-regulated by IFNG/IFN-gamma with a peak after 8 hours of treatment -
Isoforms & Post-Translational Modification
O15553 has three isomers: O15553-2: 86444 Da (predicted); O15553-1: 64494 Da (predicted); O15553-3: 50837 Da (predicted).
Cleaved by CASP1 (Probable)丨Phosphorylation at Ser-242 is required for the interaction with 14-3-3 proteins and down-regulation of pyrin pro-inflammatory activity丨Degraded along with the delivery of its substrates to autolysosomal compartments (at protein level) -
Subunit
Homotrimer
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SwissProt ID
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Synonyms
FMF antibody
Documentation
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