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SUMO2/3 Antibody

(Synonyms: SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B)

SUMO2/3 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SUMO2/3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS(pH 7.3), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SUMO2/3 Antibody
  • SUMO2/3 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
Predicted band size Info
 
Observed band size Info
 
Dilution Ratio 10/11 kD 17 kD

Product Details

Description

SUMO2/3 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SUMO2/3.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Pig,Zebrafish
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 17 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 10/11 kD
Immunogen

Synthetic peptide of human SUMO2/3.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH 7.3), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SUMO2/3 Antibody
    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), A549 (lane 3), K562 (lane 4), NIH/3T3 (lane 5), HeLa (lane 6), THP-1 (lane 7), and Mouse testis (lane 8) using SUMO2/3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • SwissProt ID

    P84022

  • Synonyms

    SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B

SUMO2/3 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), A549 (lane 3), K562 (lane 4), NIH/3T3 (lane 5), HeLa (lane 6), THP-1 (lane 7), and Mouse testis (lane 8) using SUMO2/3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SUMO2/3 Antibody Product Image
    1/16
  • Experimental Validation Results for SUMO2/3 Antibody
  • SUMO2/3 Antibody Product Image
  • Experimental Validation Results for SUMO2/3 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), A549 (lane 3), K562 (lane 4), NIH/3T3 (lane 5), HeLa (lane 6), THP-1 (lane 7), and Mouse testis (lane 8) using SUMO2/3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for SUMO2/3 Antibody
  • SUMO2/3 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), A549 (lane 3), K562 (lane 4), NIH/3T3 (lane 5), HeLa (lane 6), THP-1 (lane 7), and Mouse testis (lane 8) using SUMO2/3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SUMO2/3 Antibody Product Image WB