ATF7 Antibody
(Synonyms: ATFA, ATF7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A)ATF7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ATF7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
ATF7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ATF7.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 51 kDa
Synthetic peptide corresponding to the center region of human ATF7.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ATF7 is a Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation. In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes (By similarity). Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation (By similarity). In response to TNF, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening. Also plays a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair (By similarity)[1].
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Subcellular Localization
Nucleus; Nucleus, nucleoplasm; Chromosome, telomere
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Expression
Tissue_Specificity: Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. -
Isoforms & Post-Translational Modification
ATF7 has 6 isoforms, P17544-6: amino acid length is 483, molecular weight is 51757 Da (predicted); P17544-1: amino acid length is 494, molecular weight is 52967 Da (predicted); P17544-2: amino acid length is 462, molecular weight is 49650 Da (predicted); P17544-3: amino acid length is 473, molecular weight is 50860 Da (predicted); P17544-4: amino acid length is 307, molecular weight is 33681 Da (predicted); P17544-5: amino acid length is 117, molecular weight is 13278 Da (predicted).受 EGF 刺激时，首先在第 53 位苏氨酸(Thr-53)上发生磷酸化，随后第 51 位苏氨酸(Thr-51)发生磷酸化。Thr-51 的磷酸化会抑制 SUMO 化，增强与 TAF12 的结合，并提升转录活性ATF7 存在 6 个异构体，P17544-6：氨基酸个数为 483 个，分子量为 51757 Da (预测)；P17544-1：氨基酸个数为 494 个，分子量为 52967 Da (预测)；P17544-2：氨基酸个数为 462 个，分子量为 49650 Da (预测)；P17544-3：氨基酸个数为 473 个，分子量为 50860 Da (预测)；P17544-4：氨基酸个数为 307 个，分子量为 33681 Da (预测)；P17544-5：氨基酸个数为 117 个，分子量为 13278 Da (预测)。On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity
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Subunit
Homodimer; binds DNA as homodimer.
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SwissProt ID
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Synonyms
ATFA, ATF7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A
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