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Endo G Antibody (YA1529)

(Synonyms: EndoG; EndonucleaseG; Mitochondrial endonuclease G; NUCG_HUMAN)
Customer Review

Based on 1 Customer Validation

Endo G Antibody (YA1529) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Endo G.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    1.Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
    2.Supplied in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Endo G Antibody (YA1529) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Endo G Antibody (YA1529) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Endo G.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Endo G aa250-297/297.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    1.Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
    2.Supplied in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Endo G Antibody (YA1529)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), K562 (lane 3, 15 μg), Mouse liver (lane 4, 15 μg), and Rat liver (lane 5, 15 μg) using Endo G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Endo G Antibody (YA1529)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), HL-60 (lane 3(20μg), HUVEC (lane 4(20μg), Raji (lane 5(20μg) using Endo G Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Endo G is an Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication[1][2][3].

  • Subcellular Localization

    Mitochondrion

  • Subunit

    Homodimer; disulfide-linked (By similarity). Homodimerization is essential for enzyme activity (By similarity). Interacts with YWHAG (PubMed:33473107)

  • SwissProt ID

    Q14249

  • Gene ID
    2021 [NCBI]
  • Synonyms

    EndoG; EndonucleaseG; Mitochondrial endonuclease G; NUCG_HUMAN

  • Research Field

    Cell Biology

Endo G Antibody (YA1529) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), K562 (lane 3, 15 μg), Mouse liver (lane 4, 15 μg), and Rat liver (lane 5, 15 μg) using Endo G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), HL-60 (lane 3(20μg), HUVEC (lane 4(20μg), Raji (lane 5(20μg) using Endo G Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Endo G Antibody (YA1529) Product Image
    1/16
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Endo G Antibody (YA1529) Product Image
  • Experimental Validation Results for Endo G Antibody (YA1529)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), K562 (lane 3, 15 μg), Mouse liver (lane 4, 15 μg), and Rat liver (lane 5, 15 μg) using Endo G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Endo G Antibody (YA1529)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), HL-60 (lane 3(20μg), HUVEC (lane 4(20μg), Raji (lane 5(20μg) using Endo G Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Experimental Validation Results for Endo G Antibody (YA1529)
  • Endo G Antibody (YA1529) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), K562 (lane 3, 15 μg), Mouse liver (lane 4, 15 μg), and Rat liver (lane 5, 15 μg) using Endo G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Endo G Antibody (YA1529) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), HL-60 (lane 3(20μg), HUVEC (lane 4(20μg), Raji (lane 5(20μg) using Endo G Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Endo G Antibody (YA1529) Product Image WB
  • Endo G Antibody (YA1529) Product Image WB