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Cadherin 10 Antibody (YA2764)

(Synonyms: T2-cadherin; CDH10)
Customer Review

Based on 1 Customer Validation

Cadherin 10 Antibody (YA2764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cadherin 10.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Cadherin 10 Antibody (YA2764) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:10-1:100

Product Details

Description

Cadherin 10 Antibody (YA2764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cadherin 10.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human cadherin 10

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2), Mouse brain (lane 3), Rat kidney (lane 4), and Mouse heart (lane 5) using Cadherin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 40μg), U2OS (lane5, 20μg) and C6 (lane6, 20μg) using Cadherin 10 Antibody (HY-P83019). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Cadherin 10 Antibody (HY-P83019, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Cadherin 10 is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Predominantly expressed in brain. Also found in human kidney. Very low levels detected in prostate and fetal lung

  • SwissProt ID

    Q9Y6N8

  • Gene ID
    1008 [NCBI]
  • Synonyms

    T2-cadherin; CDH10

  • Research Field

    Cell Biology

Cadherin 10 Antibody (YA2764) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2), Mouse brain (lane 3), Rat kidney (lane 4), and Mouse heart (lane 5) using Cadherin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 40μg), U2OS (lane5, 20μg) and C6 (lane6, 20μg) using Cadherin 10 Antibody (HY-P83019). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Cadherin 10 Antibody (HY-P83019, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cadherin 10 Antibody (YA2764) Product Image
    1/16
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Cadherin 10 Antibody (YA2764) Product Image
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2), Mouse brain (lane 3), Rat kidney (lane 4), and Mouse heart (lane 5) using Cadherin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 40μg), U2OS (lane5, 20μg) and C6 (lane6, 20μg) using Cadherin 10 Antibody (HY-P83019). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Cadherin 10 Antibody (HY-P83019, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Experimental Validation Results for Cadherin 10 Antibody (YA2764)
  • Cadherin 10 Antibody (YA2764) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2), Mouse brain (lane 3), Rat kidney (lane 4), and Mouse heart (lane 5) using Cadherin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Cadherin 10 Antibody (YA2764) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y (lane2, 40μg), Rat brain tissue (lane3, 40μg), Mouse brain tissue (lane4, 40μg), U2OS (lane5, 20μg) and C6 (lane6, 20μg) using Cadherin 10 Antibody (HY-P83019). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Cadherin 10 Antibody (YA2764) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling Cadherin 10 Antibody (HY-P83019, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cadherin 10 Antibody (YA2764) Product Image WB
  • Cadherin 10 Antibody (YA2764) Product Image WB
  • Cadherin 10 Antibody (YA2764) Product Image FC