GRK6 Antibody (YA2952)
(Synonyms: GPRK6)GRK6 Antibody (YA2952) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRK6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
GRK6 Antibody (YA2952) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRK6.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 66 kDa
Entrez Gene: 2870 Human ; 26385 Mouse ;
SwissProt: P43250 Human ; O70293 Mouse ; P97711 Rat
OMIM: 600869 Human
A synthetic peptide of human GRK6 aa97-110.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GRK6 is a serine/threonine G protein-coupled receptor kinase that phosphorylates activated GPCRs, promotes β-arrestin recruitment, and limits receptor signaling[1]. Mechanistically, GRK6 regulates immune-cell chemotaxis through receptor desensitization and trafficking, especially in CXCR2, CXCR4, and LTB4/BLT1 signaling models[2][3][4]. In inflammatory models, GRK6 deficiency increases acute inflammation, enhances LTB4-induced neutrophil chemotaxis, and impairs G-CSF-induced neutrophil mobilization through enhanced CXCR4-mediated chemotaxis[3][4]. GRK6 also supports apoptotic cell clearance by cooperating with GIT1 to activate Rac1, linking this kinase to autoimmune disease mechanisms in mice[5]. Compared with related isoforms, GRK6 differs from GRK2 because CXCR1 predominantly couples to GRK2, whereas CXCR2 interacts with GRK6 to regulate sensitization, trafficking, and neutrophil function[2]. Compared with GRK5, GRK6 shows shared receptor-regulatory activity in some systems, but combinatorial knockout studies demonstrate cell-type-specific and GPCR-type-specific functions across neutrophils, T cells, B cells, and dendritic cells[6]. For experimental applications, GRK6 knockout, siRNA depletion, and overexpression models provide practical tools to study GPCR desensitization, β-arrestin signaling, immune migration, inflammation, and receptor-specific isoform selectivity[2][6][7].
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Subcellular Localization
Membrane; Lipid-anchor
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Expression
Tissue_specificity:Widely expressed -
Isoforms & Post-Translational Modification
P43250 has 3 isomers: P43250-1: 65991 Da (predicted); P43250-2: 67257 Da (predicted); P43250-3: 64294 Da (predicted).
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565 -
Subunit
Interacts with GIT1
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SwissProt ID
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Synonyms
GPRK6
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Research Field
Signal Transduction
Documentation
[1]. Krupnick JG, et al. The role of receptor kinases and arrestins in G protein-coupled receptor regulation. Annu Rev Pharmacol Toxicol. 1998;38:289-319. [Content Brief]
[2]. Fiorenza S, et al. A combination of local inflammation and central memory T cells potentiates immunotherapy in the skin. J Immunol. 2012 Dec 15;189(12):5622-31. [Content Brief]
[3]. Kavelaars A, et al. Increased acute inflammation, leukotriene B4-induced chemotaxis, and signaling in mice deficient for G protein-coupled receptor kinase 6. J Immunol. 2003 Dec 1;171(11):6128-34. [Content Brief]
[4]. Vroon A, et al. GRK6 deficiency is associated with enhanced CXCR4-mediated neutrophil chemotaxis in vitro and impaired responsiveness to G-CSF in vivo. J Leukoc Biol. 2004 Apr;75(4):698-704. [Content Brief]
[5]. Nakaya M, et al. GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance. Nat Commun. 2013;4:1532. [Content Brief]
[6]. Glaser KM, et al. Combinatorial depletions of G-protein coupled receptor kinases in immune cells identify pleiotropic and cell type-specific functions. Front Immunol. 2022 Nov 14;13:1039803. [Content Brief]
[7]. Burns RN, et al. Mechanisms of homologous and heterologous phosphorylation of FFA receptor 4 (GPR120): GRK6 and PKC mediate phosphorylation of Thr³⁴⁷, Ser³⁵⁰, and Ser³⁵⁷ in the C-terminal tail. Biochem Pharmacol. 2014 Feb 15;87(4):650-9. [Content Brief]
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