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Indoleamine 2,3-dioxygenase Antibody (YA3100)

(Synonyms: 3-dioxygenase; IDO 1; IDO; IDO1; INDO; indolamine 2; 3 dioxygenase; Indole 2 3 dioxygenase)
Customer Review

Based on 1 Customer Validation

Indoleamine 2,3-dioxygenase Antibody (YA3100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Indoleamine 2,3-dioxygenase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

Indoleamine 2,3-dioxygenase Antibody (YA3100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Indoleamine 2,3-dioxygenase.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database

Entrez Gene: 3620 Human

SwissProt: P14902 Human

Immunogen

Recombinant protein of human Indoleamine 2,3-dioxygenase aa1-403.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105468

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    Western blot analysis of extracts from Hela(lane 2(20ug)，Hela(lane 3(40ug) using Indoleamine 2,3-dioxygenase Antibody (HY-P83355) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    Immunocytochemistry analysis of HUVEC cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    Immunocytochemistry analysis of Hela cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Indoleamine 2,3-dioxygenase catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla) , in some epithelial cells of the female genital tract, as well as in endothelial cells of term placenta and in lung parenchyma (PubMed:25691885) . Weakly or not expressed in most normal tissues, but mostly inducible in most tissues (PubMed:25691885) . Expressed in more than 50% of tumors, either by tumoral, stromal, or endothelial cells (expression in tumor is associated with a worse clinical outcome) (PubMed:18418598) . Not overexpressed in tumor-draining lymph nodes (PubMed:25691885, PubMed:26155395)

    Induction:By IFNG/IFN-gamma in most cells (PubMed:1907934, PubMed:2109605) . Exogenous inflammatory stimuli induce the expression of IDO1 in antigen-presenting cells such as dendritic cells, macrophages and B-cells (PubMed:25157255) .

  • Subunit

    Monomer

  • SwissProt ID

    P14902

  • Gene ID
    3620 [NCBI]
  • Synonyms

    3-dioxygenase; IDO 1; IDO; IDO1; INDO; indolamine 2; 3 dioxygenase; Indole 2 3 dioxygenase

  • Research Field

    Signal Transduction

Indoleamine 2,3-dioxygenase Antibody (YA3100) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，Hela(lane 3(40ug) using Indoleamine 2,3-dioxygenase Antibody (HY-P83355) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
    1/16
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，Hela(lane 3(40ug) using Indoleamine 2,3-dioxygenase Antibody (HY-P83355) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Experimental Validation Results for Indoleamine 2,3-dioxygenase Antibody (YA3100)
  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug)，Hela(lane 3(40ug) using Indoleamine 2,3-dioxygenase Antibody (HY-P83355) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HUVEC cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Indoleamine 2,3-dioxygenase Antibody (HY-P83355) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image WB
  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image ICC
  • Indoleamine 2,3-dioxygenase Antibody (YA3100) Product Image ICC