AHA1 Antibody
(Synonyms: AHSA1, C14orf3, HSPC322, Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38)
Based on 1 Customer Validation
AHA1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to AHA1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IF-Tissue, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:100-1:300 | 1:5000-20000 | 1:50-200 |
Product Details
AHA1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to AHA1.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse
The antiserum was produced against synthesized peptide derived from human AHSA1.AA range:161-210.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
AHA1 acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins[1][2].
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Subcellular Localization
Cytoplasm, cytosol; Endoplasmic reticulum
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Expression
Tissue_Specificity: Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
Induction: By heat shock and treatment with the HSP90 inhibitor 17-demethoxygeldanamycin (17AAG). -
Isoforms & Post-Translational Modification
AHA1 has 2 isoforms, O95433-1: amino acid length is 338, molecular weight is 38274 Da (predicted); O95433-2: amino acid length is 288, molecular weight is 32340 Da (predicted).
Phosphorylation at Tyr-223 enhances binding to chaperone HSP90AA1. -
Subunit
Interacts with HSPCA/HSP90.
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SwissProt ID
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Synonyms
AHSA1, C14orf3, HSPC322, Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38
Documentation
[1]. Woodford MR, et al. Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients. EMBO J. 2017 Dec 15;36(24):3650-3665. [Content Brief]
[2]. Woodford MR, et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nat Commun. 2016 Jun 29;7:12037. [Content Brief]
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