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CD44 Antibody (YA801)

(Synonyms: CD44; LHR; MDU2; MDU3; MIC4; CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycopr)
Customer Review

Based on 1 Customer Validation

CD44 Antibody (YA801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD44.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • CD44 Antibody (YA801) Product Image
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

CD44 Antibody (YA801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD44.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 82 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Species Reactivity Database

Entrez Gene: 960 Human

SwissProt: P16070 Human

Immunogen

Synthetic peptide corresponding to Human CD44.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102623

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD44 Antibody (YA801)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) ,HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody (HY-P80602) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA801)
    Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P80602) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD44 Antibody (YA801)
    Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CD44 is a non-kinase cell surface transmembrane glycoprotein. It was cloned and classified as a member of the cartilage junction protein family. CD44 is considered to be the major cell surface receptor for hyaluronan, a major extracellular matrix (ECM) component. CD44 is prominently expressed on lymphocytes, smooth muscle, fibroblasts, and various types of epithelium and is involved in lymphocyte homing, cell adhesion and aggregation, cell migration, leukocyte activation, lymphopoiesis and myelopoiesis, angiogenesis, and cytokine release.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, microvillus; Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in fibroblasts and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . Protein levels were also detected in the placenta (PubMed:32337544) . Isomer 10 (epithelial isomer) is expressed in epithelial cells and is highly expressed in cancer cells. Expression is suppressed in neuroblastoma cells.

  • Isoforms & Post-Translational Modification

    P16070 has 19 isomers: P16070-1: 81538 Da (predicted); P16070-2: 3327 Da (predicted); P16070-3: 77983 Da (predicted); P16070-4: 76612 Da (predicted); P16070-5: 80790 Da (predicted); P16070-6: 76705 Da (predicted); P16070-7: 78446 Da (predicted); P16070-8: 74388 Da (predicted); P16070-9: 74196 Da (predicted); P16070-10: 53411 Da (predicted); P16070-11: 46565 Da (predicted); P16070-12: 39416 Da (predicted); P16070-13: 46261 Da (predicted); P16070-14: 43169 Da (predicted); P16070-15: 32075 Da (predicted); P16070-16: 73150 Da (predicted); P16070-17: 75957 Da (predicted); P16070-18: 37278 Da (predicted); P16070-19: 15635 Da (predicted).
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors;N-glycosylated;O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638;Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672

  • Subunit

    Interacts with PKN2 (PubMed:15123640). Interacts with TIAM1 and TIAM2 (By similarity). Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment (PubMed:14992719, PubMed:17085435, PubMed:25195884). Interacts with UNC119 (PubMed:19381274). Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration (PubMed:20962267). Interacts with RDX, EZR and MSN (By similarity). Interacts with EGFR (PubMed:18757307, PubMed:23589287). Interacts with CD74; this complex is essential for the MIF-induced signaling cascade that results in B cell survival (By similarity)

  • SwissProt ID

    P16070

  • Gene ID
    960 [NCBI]
  • Synonyms

    CD44; LHR; MDU2; MDU3; MIC4; CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycopr

  • Research Field

    Immunology

CD44 Antibody (YA801) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) ,HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody (HY-P80602) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P80602) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD44 Antibody (YA801) Product Image
    1/16
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • CD44 Antibody (YA801) Product Image
  • Experimental Validation Results for CD44 Antibody (YA801)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) ,HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody (HY-P80602) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA801)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P80602) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD44 Antibody (YA801)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • Experimental Validation Results for CD44 Antibody (YA801)
  • CD44 Antibody (YA801) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) ,HUVEC(lane 3(20ug) and J774A.1(lane 4(20ug) using CD44 Antibody (HY-P80602) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD44 Antibody (YA801) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P80602) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD44 Antibody (YA801) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling CD44 with CD44 antibody (HY-P80602) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD44 antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD44 Antibody (YA801) Product Image WB
  • CD44 Antibody (YA801) Product Image ICC
  • CD44 Antibody (YA801) Product Image ICC