Human IgG4 Antibody (YA3327)(PBS only)

Cat. No.: HY-P83582A: Data Sheet User Guide for Antibodies
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Human IgG4 Antibody (YA3327) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Human IgG4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Human IgG4 Antibody (YA3327) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Human IgG4.

Host

Rabbit

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P01861

Gene ID

3503 [NCBI]

Immunogen

A synthesized peptide derived from human IgG4 aa95-109.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268)

Subcellular Localization：Secreted; Cell membrane; Single-pass membrane protein

Isoforms & Post-Translational Modification：P01861 has 2 isomers: P01861-2: 43832 Da (predicted); P01861-1: 35941 Da (predicted).
Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway;(Microbial infection) Deglycosylation on Asn-177 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway

Subunit：Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked

Research Field

Immunology

Synonyms

IGG4; Ig gamma 4 chain C region

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mt4-cell.html [Content Brief]