CHD4 Antibody (YA947)
(Synonyms: CHD4; Chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta)CHD4 Antibody (YA947) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:50-1:100 |
Product Details
CHD4 Antibody (YA947) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD4.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 260 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 218 kDa
A synthesized peptide derived from human CHD4 aa1800-1870/1912.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CHD4 is an ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_specificity:Broad expression -
Isoforms & Post-Translational Modification
Q14839 has 2 isomers: Q14839-1: 218005 Da (predicted); Q14839-2: 220848 Da (predicted).
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Subunit
Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:10204490, PubMed:15454082, PubMed:16428440, PubMed:28977666, PubMed:33283408, PubMed:9804427).
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SwissProt ID
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Synonyms
CHD4; Chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
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Research Field
Immunology
Documentation
[1]. Hoffmeister H, et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res. 2017 Oct 13;45(18):10534-10554. [Content Brief]
[2]. Farnung L, et al. Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations. Elife. 2020 Jun 16;9:. [Content Brief]
[3]. Le Guezennec X, et al. MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties. Mol Cell Biol. 2006 Feb;26(3):843-51. [Content Brief]
[4]. Sillibourne JE, et al. Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Mol Biol Cell. 2007 Sep;18(9):3667-80. [Content Brief]
[5]. Tong JK, et al. Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex. Nature. 1998 Oct 29;395(6705):917-21. [Content Brief]
[6]. Gong F, et al. Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Genes Dev. 2015 Jan 15;29(2):197-211. [Content Brief]
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