Copy Link Print Information

MT ND1 Antibody (YA2089)

(Synonyms: MT-ND1; MTND1; NADH1; ND1; NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

MT ND1 Antibody (YA2089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MT ND1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MT ND1 Antibody (YA2089)
  • MT ND1 Antibody (YA2089) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) MT ND1 Antibody (YA2089)

More

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MT ND1 Antibody (YA2089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MT ND1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MT-ND1 aa15-80/318.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104453

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MT ND1 Antibody (YA2089)
    Western blot analysis of extracts from Rat heart(lane 2(20μg), Mouse heart(lane 3(20μg) ,Rat skeletal muscle(lane 4(20μg) and Mouse skeletal muscle( lane 5(20μg) using MT ND1 Antibody (HY-P82344). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MT ND1 core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I[1][2].

  • Subcellular Localization

    Mitochondrion inner membrane; Multi-pass membrane protein

  • Subunit

    Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits

  • SwissProt ID

    P03886

  • Gene ID
    4535 [NCBI]
  • Synonyms

    MT-ND1; MTND1; NADH1; ND1; NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1

  • Research Field

    Signal Transduction

MT ND1 Antibody (YA2089) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart(lane 2(20μg), Mouse heart(lane 3(20μg) ,Rat skeletal muscle(lane 4(20μg) and Mouse skeletal muscle( lane 5(20μg) using MT ND1 Antibody (HY-P82344). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • MT ND1 Antibody (YA2089) Product Image
    1/16
  • Experimental Validation Results for MT ND1 Antibody (YA2089)
  • MT ND1 Antibody (YA2089) Product Image
  • Experimental Validation Results for MT ND1 Antibody (YA2089)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart(lane 2(20μg), Mouse heart(lane 3(20μg) ,Rat skeletal muscle(lane 4(20μg) and Mouse skeletal muscle( lane 5(20μg) using MT ND1 Antibody (HY-P82344). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MT ND1 Antibody (YA2089)
  • MT ND1 Antibody (YA2089) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat heart(lane 2(20μg), Mouse heart(lane 3(20μg) ,Rat skeletal muscle(lane 4(20μg) and Mouse skeletal muscle( lane 5(20μg) using MT ND1 Antibody (HY-P82344). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • MT ND1 Antibody (YA2089) Product Image WB