Phospho-Parvin alpha (Ser8) Antibody (YA1539)(PBS only)

Cat. No.: HY-P81794A: Data Sheet User Guide for Antibodies
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Phospho-Parvin alpha (Ser8) Antibody (YA1539) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Parvin alpha (Ser8).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-Parvin alpha (Ser8) Antibody (YA1539) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Parvin alpha (Ser8).

Host

Rabbit

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NVD7

Gene ID

55742 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser8 of human Parvin alpha

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a role in sarcomere organization and in smooth muscle cell contraction. Required for normal development of the embryonic cardiovascular system, and for normal septation of the heart outflow tract. Plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development (By similarity). Plays a role in the reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. Plays a role in the establishment of cell polarity, cell adhesion, cell spreading, and directed cell migration. Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed:30367047)

Subcellular Localization：Cell junction, focal adhesion; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Cytoplasm, myofibril, sarcomere, Z line

Expression：
Tissue_specificity:Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver

Isoforms & Post-Translational Modification：Q9NVD7 has 2 isomers: Q9NVD7-1: 42244 Da (predicted); Q9NVD7-2: 20625 Da (predicted).

Subunit：Component of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex composed of ILK, LIMS1/PINCH and PARVA; the complex binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling (PubMed:11331308, PubMed:12432066, PubMed:15284246, PubMed:15817463, PubMed:20005845, PubMed:30367047, PubMed:35259013). Formation of the IPP complex is dependent on protein kinase C and precedes integrin-mediated cell adhesion and spreading (PubMed:12432066). Interacts with TGFB1I1 (By similarity). Interacts with ARHGAP31 (PubMed:16860736). Interacts with the actin cytoskeleton (PubMed:11331308). Interacts (via C-terminus) with TESK1 (via C-terminus); the interaction inhibits TESK1 kinase activity (PubMed:15817463). Interacts with PXN/PAXILLIN (via LD motif 4) (PubMed:15817463, PubMed:18508764, PubMed:18940607)

Research Field

Synonyms

MXRA2; CH-ILKBP

Documentation

User Guide for Antibodies (1077 KB)